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Magnetic Luminex Assay Kit for Fibroblast Growth Factor 10 (FGF10) ,etc.
Keratinocyte growth factor 2
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB882Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.98-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.327 pg/mL.
- DownloadInstruction Manual
- UOM 1Plex2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
- FOB
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Specificity
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 10 (FGF10) ,etc..
No significant cross-reactivity or interference between Fibroblast Growth Factor 10 (FGF10) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 10 (FGF10) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 10 (FGF10) ,etc. in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 78-90 | 87 |
EDTA plasma(n=5) | 88-95 | 92 |
heparin plasma(n=5) | 91-105 | 96 |
sodium citrate plasma(n=5) | 94-104 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 10 (FGF10) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 10 (FGF10) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 10 (FGF10) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 91-105% | 80-94% | 91-101% | 99-105% |
EDTA plasma(n=5) | 98-105% | 85-99% | 85-97% | 98-105% |
heparin plasma(n=5) | 91-105% | 81-103% | 84-91% | 93-105% |
sodium citrate plasma(n=5) | 88-102% | 97-104% | 81-102% | 99-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:FGF10) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Inflammation | Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair. pubmed:28540539 |
Journal of Molecular Neuroscience | Dysregulation of Fibroblast Growth Factor 10 in the Peripheral Blood of Patients with Schizophrenia Pubmed: 31256336 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPB882Hu01 | Recombinant Fibroblast Growth Factor 10 (FGF10) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPB882Hu61 | Eukaryotic Fibroblast Growth Factor 10 (FGF10) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB882Hu01 | Active Fibroblast Growth Factor 10 (FGF10) | Cell culture; Activity Assays. |
PAB882Hu06 | Polyclonal Antibody to Fibroblast Growth Factor 10 (FGF10) | WB; IHC; ICC; IP. |
PAB882Hu01 | Polyclonal Antibody to Fibroblast Growth Factor 10 (FGF10) | WB; IHC; ICC; IP. |
MAB882Hu22 | Monoclonal Antibody to Fibroblast Growth Factor 10 (FGF10) | WB; IHC; ICC; IP. |
SEB882Hu | ELISA Kit for Fibroblast Growth Factor 10 (FGF10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB882Hu | CLIA Kit for Fibroblast Growth Factor 10 (FGF10) | Chemiluminescent immunoassay for Antigen Detection. |
LMB882Hu | Magnetic Luminex Assay Kit for Fibroblast Growth Factor 10 (FGF10) ,etc. | Magnetic Luminex Assay for Antigen Detection. |