Multiplex Assay Kit for Exportin 1 (XPO1) ,etc. by FLIA (Flow Luminescence Immunoassay)

Exp1; CRM1 Homolog,Yeast; Chromosome region maintenance 1 protein homolog

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Exportin 1 (XPO1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Exportin 1 (XPO1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Exportin 1 (XPO1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Exportin 1 (XPO1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-102 94
EDTA plasma(n=5) 82-95 86
heparin plasma(n=5) 87-101 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Exportin 1 (XPO1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Exportin 1 (XPO1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Exportin 1 (XPO1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-103% 98-105% 79-103% 80-97%
EDTA plasma(n=5) 83-102% 85-95% 92-105% 82-101%
heparin plasma(n=5) 91-99% 79-95% 78-92% 96-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:XPO1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Clin Cancer Res Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402
Clin Cancer Res Inactivation of the p53–KLF8–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402
Cellular Oncology Mahendra Seervi, S. Sumi, Aneesh Chandrasekharan, Abhay K. Sharma & Doi: 10.1007/s13402-019-00451-1
Cellular Oncology Molecular profiling of anastatic cancer cells: potential role of the nuclear export pathway Pubmed: 31147963
Journal of Cardiovascular Translational Research XPO1 Gene Therapy Attenuates Cardiac Dysfunction in Rats with Chronic Induced Myocardial Infarction Pubmed: 31768947
Biol Chem Identification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry 34472763
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC258Hu01 Recombinant Exportin 1 (XPO1) Positive Control; Immunogen; SDS-PAGE; WB.
PAC258Hu01 Polyclonal Antibody to Exportin 1 (XPO1) WB,IHC; FCM
FAC258Hu01 Anti-Exportin 1 (XPO1) Polyclonal Antibody Flow cytometry.
FAC258Hu51 APC-Linked Anti-Exportin 1 (XPO1) Polyclonal Antibody Flow cytometry.
MAC258Hu21 Monoclonal Antibody to Exportin 1 (XPO1) WB; IHC; ICC; IP.
SEC258Hu ELISA Kit for Exportin 1 (XPO1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC258Hu Multiplex Assay Kit for Exportin 1 (XPO1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.