Insulin (INS)

Insulin is a polypeptide hormone originating in the beta cells of the pancreas and serving as a principal regulator for the storage and production of carbohydrates. Its secretion is normally stimulated by increases in the amount of glucose in circulation. This leads to higher insulin levels and more rapid tissueassimilation of glucose followed by a decline in the insulin level as the glucose level subsides. In a number of conditions, notably insulinoma and diabetes, this relationship is impaired. Insulin tends to circulate at inappropriately high levels in patients with insulin-secreting pancreatic tumors; such tumors can thus be a cause of hypoglycemia. Accordingly, insulin immunoassays used sometimes in connection with provocative doses of tolbutamide or calcium play an essential role in the identification (and localization) of insulinomas.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA448Hu01Recombinant Insulin (INS)SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
NPA448Hu01Native Insulin (INS)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA448Hu01Polyclonal Antibody to Insulin (INS)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
MAA448Hu22Monoclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
LAA448Hu71Biotin-Linked Polyclonal Antibody to Insulin (INS)IHC;WB;ELISA
LAA448Hu81FITC-Linked Polyclonal Antibody to Insulin (INS)IHC;ICC;IF;WB
PAA448Hu02Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsCEA448HuELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay for Antigen Detection
CCA448HuCLIA Kit for Insulin (INS)Chemiluminescent immunoassay for Antigen Detection

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA448Mu01Recombinant Insulin (INS)SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
RPA448Mu02Recombinant Insulin (INS)SDS-PAGE; WB; ELISA; IP.
APA448Mu01Active Insulin (INS)Cell culture; Activity Assays; In vivo assays.
AntibodiesPAA448Mu01Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
PAA448Mu02Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsCEA448MuELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA448Ra01Recombinant Insulin (INS)SDS-PAGE; WB; ELISA; IP.
NPA448Ra01Native Insulin (INS)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA448Ra01Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
MAA448Ra21Monoclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsCEA448RaELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay for Antigen Detection
CCA448RaCLIA Kit for Insulin (INS)Chemiluminescent immunoassay for Antigen Detection

Organism species: Cavia (Guinea pig )

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA448Gu01Recombinant Insulin (INS)SDS-PAGE; WB; ELISA; IP.
CPA448Gu21OVA Conjugated Insulin (INS)Immunogen; Coating Antigen; Blocking Peptide; ELISA.
AntibodiesPAA448Gu01Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
MAA448Gu21Monoclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
PAA448Gu08Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsCEA448GuELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA448Rb01Recombinant Insulin (INS)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA448Rb51Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsCEA448RbELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Insulin (INS)Recombinant Protein Customized Service Offer
AntibodiesPAA448Si01Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsCEA448SiELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Insulin (INS)Recombinant Protein Customized Service Offer
AntibodiesPAA448Ca01Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA448CaELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay for Antigen Detection
CEA448CaELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA448Po01Recombinant Insulin (INS)SDS-PAGE; WB; ELISA; IP
RPA448Po02Recombinant Insulin (INS)SDS-PAGE; WB; ELISA; IP.
CPA448Po21OVA Conjugated Insulin (INS)Immunogen; Coating Antigen; Blocking Peptide; ELISA.
AntibodiesPAA448Po01Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
PAA448Po02Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsCEA448PoELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA448Bo01Recombinant Insulin (INS)SDS-PAGE; WB; ELISA; IP.
CPA448Bo21OVA Conjugated Insulin (INS)Immunogen; Coating Antigen; Blocking Peptide; ELISA.
AntibodiesPAA448Bo01Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
LAA448Bo71Biotin-Linked Polyclonal Antibody to Insulin (INS)IHC;WB;ELISA
MAA448Bo21Monoclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsCEA448BoELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay for Antigen Detection
  1. "Sequence of the human insulin gene."Nature 284:26-32(1980) [PubMed] [Europe PMC] [Abstract]
  2. "Genetic variation in the human insulin gene."Science 209:612-615(1980) [PubMed] [Europe PMC] [Abstract]
  3. "Nucleotide sequence of a cDNA clone encoding human preproinsulin."Nature 282:525-527(1979) [PubMed] [Europe PMC] [Abstract]
  4. "Nucleotide sequence of human preproinsulin complementary DNA."Science 208:57-59(1980) [PubMed] [Europe PMC] [Abstract]
  5. "Susceptibility to insulin dependent diabetes mellitus maps to a 4.1 kb segment of DNA spanning the insulin gene and associated VNTR."Nat. Genet. 4:305-310(1993) [PubMed] [Europe PMC] [Abstract]
  6. "Insulinomas and expression of an insulin splice variant."Lancet 363:363-367(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Global haplotype diversity in the human insulin gene region."Genome Res. 13:2101-2111(2003) [PubMed] [Europe PMC] [Abstract]
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  9. "Amino-acid sequence of human insulin."Nature 187:483-485(1960) [PubMed] [Europe PMC] [Abstract]
  10. "Studies on human proinsulin. Isolation and amino acid sequence of the human pancreatic C-peptide."J. Biol. Chem. 246:1375-1386(1971) [PubMed] [Europe PMC] [Abstract]
  11. "The amino acid sequence of the C-peptide of human proinsulin."Eur. J. Biochem. 20:190-199(1971) [PubMed] [Europe PMC] [Abstract]
  12. "Total synthesis of human insulin under directed formation of the disulfide bonds."Helv. Chim. Acta 57:2617-2621(1974) [PubMed] [Europe PMC] [Abstract]
  13. "Studies on polypeptides, IV. The synthesis of C-peptide of human proinsulin."Hoppe-Seyler's Z. Physiol. Chem. 354:659-672(1973) [PubMed] [Europe PMC] [Abstract]
  14. "Synthesis of peptides with the properties of human proinsulin C peptides (hC peptide). 3. Synthesis of the sequences 14-17 and 9-13 of human proinsulin C peptides."Chem. Ber. 106:199-205(1973) [PubMed] [Europe PMC] [Abstract]
  15. "Synthesis of peptides with the properties of human proinsulin C peptides (hC peptide). I. Scheme for the synthesis and preparation of the sequence 28-31 of human proinsulin C peptide."Chem. Ber. 106:188-192(1973) [PubMed] [Europe PMC] [Abstract]
  16. "Studies on mutant human insulin genes: identification and sequence analysis of a gene encoding [SerB24]insulin."Proc. Natl. Acad. Sci. U.S.A. 80:6366-6370(1983) [PubMed] [Europe PMC] [Abstract]
  17. "Identification of a mutant human insulin predicted to contain a serine-for-phenylalanine substitution."Proc. Natl. Acad. Sci. U.S.A. 80:7390-7394(1983) [PubMed] [Europe PMC] [Abstract]
  18. "A mutation in the B chain coding region is associated with impaired proinsulin conversion in a family with hyperproinsulinemia."Proc. Natl. Acad. Sci. U.S.A. 84:2194-2197(1987) [PubMed] [Europe PMC] [Abstract]
  19. "Structurally abnormal insulin in a diabetic patient. Characterization of the mutant insulin A3 (Val-->Leu) isolated from the pancreas."J. Clin. Invest. 78:1666-1672(1986) [PubMed] [Europe PMC] [Abstract]
  20. "Two unrelated patients with familial hyperproinsulinemia due to a mutation substituting histidine for arginine at position 65 in the proinsulin molecule: identification of the mutation by direct sequencing of genomic deoxyribonucleic acid amplified by polymerase chain reaction."J. Clin. Endocrinol. Metab. 71:164-169(1990) [PubMed] [Europe PMC] [Abstract]
  21. "Posttranslational cleavage of proinsulin is blocked by a point mutation in familial hyperproinsulinemia."J. Clin. Invest. 76:378-380(1985) [PubMed] [Europe PMC] [Abstract]
  22. "A novel point mutation in the human insulin gene giving rise to hyperproinsulinemia (proinsulin Kyoto)."J. Clin. Invest. 89:1902-1907(1992) [PubMed] [Europe PMC] [Abstract]
  23. "Toward the solution structure of human insulin: sequential 2D 1H NMR assignment of a des-pentapeptide analogue and comparison with crystal structure."Biochemistry 29:10545-10555(1990) [PubMed] [Europe PMC] [Abstract]
  24. "Comparative 2D NMR studies of human insulin and des-pentapeptide insulin: sequential resonance assignment and implications for protein dynamics and receptor recognition."Biochemistry 30:5505-5515(1991) [PubMed] [Europe PMC] [Abstract]
  25. "Two-dimensional NMR studies of Des-(B26-B30)-insulin: sequence-specific resonance assignments and effects of solvent composition."Biochim. Biophys. Acta 1078:101-110(1991) [PubMed] [Europe PMC] [Abstract]
  26. "Three-dimensional solution structure of an insulin dimer. A study of the B9(Asp) mutant of human insulin using nuclear magnetic resonance, distance geometry and restrained molecular dynamics."J. Mol. Biol. 227:1146-1163(1992) [PubMed] [Europe PMC] [Abstract]
  27. "Paradoxical structure and function in a mutant human insulin associated with diabetes mellitus."Proc. Natl. Acad. Sci. U.S.A. 90:582-586(1993) [PubMed] [Europe PMC] [Abstract]
  28. "Solution structures of the R6 human insulin hexamer."Biochemistry 36:9409-9422(1997) [PubMed] [Europe PMC] [Abstract]
  29. "Insulin gene mutations as a cause of permanent neonatal diabetes."Proc. Natl. Acad. Sci. U.S.A. 104:15040-15044(2007) [PubMed] [Europe PMC] [Abstract]
  30. "Insulin mutation screening in 1,044 patients with diabetes: mutations in the INS gene are a common cause of neonatal diabetes but a rare cause of diabetes diagnosed in childhood or adulthood."Diabetes 57:1034-1042(2008) [PubMed] [Europe PMC] [Abstract]
  31. "Mutations in the insulin gene can cause MODY and autoantibody-negative type 1 diabetes."Diabetes 57:1131-1135(2008) [PubMed] [Europe PMC] [Abstract]
  32. "Further evidence that mutations in INS can be a rare cause of Maturity-Onset Diabetes of the Young (MODY)."BMC Med. Genet. 11:42-42(2010) [PubMed] [Europe PMC] [Abstract]