Dihydrolipoyl Transacetylase (DLAT)

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DLTA; PDC-E2; PBC; M2 antigen complex 70 kDa subunit; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Pyruvate dehydrogenase complex E2

Dihydrolipoyl Transacetylase (DLAT)

Dihydrolipoyl transacetylase is the component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA696Hu01Recombinant Dihydrolipoyl Transacetylase (DLAT)SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
AntibodiesPAA696Hu01Polyclonal Antibody to Dihydrolipoyl Transacetylase (DLAT)WB, ICC, IHC-P, IHC-F, ELISA
Assay Kitsn/aCLIA Kit for Dihydrolipoyl Transacetylase (DLAT)CLIA Kit Customized Service Offer
n/aELISA Kit for Dihydrolipoyl Transacetylase (DLAT)ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA696Mu01Recombinant Dihydrolipoyl Transacetylase (DLAT)SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
RPA696Mu02Recombinant Dihydrolipoyl Transacetylase (DLAT)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA696Mu01Polyclonal Antibody to Dihydrolipoyl Transacetylase (DLAT)WB, ICC, IHC-P, IHC-F, ELISA
PAA696Mu02Polyclonal Antibody to Dihydrolipoyl Transacetylase (DLAT)WB, ICC, IHC-P, IHC-F, ELISA
LAA696Mu71Biotin-Linked Polyclonal Antibody to Dihydrolipoyl Transacetylase (DLAT)WB; ICC; IHC-P; IHC-F; IF; ELISA.
Assay KitsSEA696MuELISA Kit for Dihydrolipoyl Transacetylase (DLAT)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Dihydrolipoyl Transacetylase (DLAT)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Dihydrolipoyl Transacetylase (DLAT)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Dihydrolipoyl Transacetylase (DLAT)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Dihydrolipoyl Transacetylase (DLAT)CLIA Kit Customized Service Offer
n/aELISA Kit for Dihydrolipoyl Transacetylase (DLAT)ELISA Kit Customized Service Offer

Organism species: Danio rerio (Zebrafish)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Dihydrolipoyl Transacetylase (DLAT)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Dihydrolipoyl Transacetylase (DLAT)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Dihydrolipoyl Transacetylase (DLAT)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Dihydrolipoyl Transacetylase (DLAT)CLIA Kit Customized Service Offer
n/aELISA Kit for Dihydrolipoyl Transacetylase (DLAT)ELISA Kit Customized Service Offer
  1. "Human chromosome 11 DNA sequence and analysis including novel gene identification." Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
  2. "Primary structure of the human M2 mitochondrial autoantigen of primary biliary cirrhosis: dihydrolipoamide acetyltransferase."Proc. Natl. Acad. Sci. U.S.A. 85:7317-7321(1988) [PubMed] [Europe PMC] [Abstract]
  3. "Nucleotide sequence of a cDNA for the dihydrolipoamide acetyltransferase component of human pyruvate dehydrogenase complex."FEBS Lett. 240:45-48(1988) [PubMed] [Europe PMC] [Abstract]
  4. "Organization of the cores of the mammalian pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding protein and their capacities to bind the E1 and E3 components."J. Biol. Chem. 279:6921-6933(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  6. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  7. "Three-dimensional structure of the major autoantigen in primary biliary cirrhosis."Gastroenterology 115:139-146(1998) [PubMed] [Europe PMC] [Abstract]
  8. "Clinical and genetic spectrum of pyruvate dehydrogenase deficiency: dihydrolipoamide acetyltransferase (E2) deficiency."Ann. Neurol. 58:234-241(2005) [PubMed] [Europe PMC] [Abstract]
  9. "Crystal structure of pyruvate dehydrogenase kinase 3 bound to lipoyl domain 2 of human pyruvate dehydrogenase complex."EMBO J. 24:1763-1774(2005) [PubMed] [Europe PMC] [Abstract]
  10. "Solution structure of RSGI RUH-058, a lipoyl domain of human 2-oxoacid dehydrogenase."Submitted (OCT-2006) to the PDB data bank
  11. "Crystal structure of an asymmetric complex of pyruvate dehydrogenase kinase 3 with lipoyl domain 2 and its biological implications."J. Mol. Biol. 370:407-416(2007) [PubMed] [Europe PMC] [Abstract]
  12. "Distinct structural mechanisms for inhibition of pyruvate dehydrogenase kinase isoforms by AZD7545, dichloroacetate, and radicicol."Structure 15:992-1004(2007) [PubMed] [Europe PMC] [Abstract]
  13. "Structural and functional insights into the molecular mechanisms responsible for the regulation of pyruvate dehydrogenase kinase 2."J. Biol. Chem. 283:15789-15798(2008) [PubMed] [Europe PMC] [Abstract]