Macrophage Migration Inhibitory Factor (MIF)

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GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

Macrophage Migration Inhibitory Factor (MIF)

One of the first cytokines described, MIF (macrophage migration inhibitory factor) was originally identified in studies of delayed hypersensitivity reactions where it was shown to inhibit macrophage migration. It is an important mediator of the innate immune response with potential roles in the pathophysiology of inflammatory, autoimmune, and neoplastic disorders. The human MIF gene encodes a 115 amino acid, 12.5 kDa secreted protein. Crystallographic studies suggest that MIF exists as a homotrimer, although some reports show that it may exist as a dimer or monomer as well. Although MIF exhibits no homology with other known cytokines, it shares structural homology with several bacterial enzymes. It has been speculated that MIF is an inflammatory mediator possibly associated with rheumatoid arthritis (RA) severity.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA698Hu01 Recombinant Macrophage Migration Inhibitory Factor (MIF) Positive Control; Immunogen; SDS-PAGE; WB.
RPA698Hu02 Recombinant Macrophage Migration Inhibitory Factor (MIF) Positive Control; Immunogen; SDS-PAGE; WB.
APA698Hu01 Active Macrophage Migration Inhibitory Factor (MIF) Cell culture; Activity Assays.
Antibodies PAA698Hu01 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
MAA698Hu22 Monoclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
PAA698Hu02 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
Assay Kits SEA698Hu ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA698Mu01 Recombinant Macrophage Migration Inhibitory Factor (MIF) Positive Control; Immunogen; SDS-PAGE; WB.
RPA698Mu02 Recombinant Macrophage Migration Inhibitory Factor (MIF) Positive Control; Immunogen; SDS-PAGE; WB.
APA698Mu01 Active Macrophage Migration Inhibitory Factor (MIF) Cell culture; Activity Assays.
Antibodies PAA698Mu01 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
PAA698Mu02 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
Assay Kits SEA698Mu ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA698Mu CLIA Kit for Macrophage Migration Inhibitory Factor (MIF) Chemiluminescent immunoassay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA698Ra01 Recombinant Macrophage Migration Inhibitory Factor (MIF) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA698Ra01 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
MAA698Ra21 Monoclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
Assay Kits SEA698Ra ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rhesus monkey (Simian)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Macrophage Migration Inhibitory Factor (MIF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Macrophage Migration Inhibitory Factor (MIF) CLIA Kit Customized Service Offer
n/a ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) ELISA Kit Customized Service Offer

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Macrophage Migration Inhibitory Factor (MIF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Polyclonal Antibody Customized Service Offer
Assay Kits SEA698Po ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Macrophage Migration Inhibitory Factor (MIF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Macrophage Migration Inhibitory Factor (MIF) CLIA Kit Customized Service Offer
n/a ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) ELISA Kit Customized Service Offer

Organism species: Capra hircus; Caprine (Goat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Macrophage Migration Inhibitory Factor (MIF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Macrophage Migration Inhibitory Factor (MIF) CLIA Kit Customized Service Offer
n/a ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) ELISA Kit Customized Service Offer
  1. "Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor."Proc. Natl. Acad. Sci. U.S.A. 86:7522-7526(1989) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor."Proc. Natl. Acad. Sci. U.S.A. 90:10056-10060(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF)."Biochemistry 33:14144-14155(1994) [PubMed] [Europe PMC] [Abstract]
  4. "Cloning the human gene for macrophage migration inhibitory factor (MIF)."Genomics 19:48-51(1994) [PubMed] [Europe PMC] [Abstract]
  5. "A genome annotation-driven approach to cloning the human ORFeome."Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
  9. "Human liver protein map: a reference database established by microsequencing and gel comparison."Electrophoresis 13:992-1001(1992) [PubMed] [Europe PMC] [Abstract]
  10. "The major binding protein of the interferon antagonist sarcolectin in human placenta is a macrophage migration inhibitory factor."Arch. Biochem. Biophys. 303:74-80(1993) [PubMed] [Europe PMC] [Abstract]
  11. "A macrophage migration inhibitory factor is expressed in the differentiating cells of the eye lens."Proc. Natl. Acad. Sci. U.S.A. 90:1272-1275(1993) [PubMed] [Europe PMC] [Abstract]
  12. "Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1."Nature 408:211-216(2000) [PubMed] [Europe PMC] [Abstract]
  13. "A novel 5'-flanking region polymorphism of macrophage migration inhibitory factor is associated with systemic-onset juvenile idiopathic arthritis."Arthritis Rheum. 44:1782-1785(2001) [PubMed] [Europe PMC] [Abstract]
  14. "Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis."Biochem. J. 357:373-383(2001) [PubMed] [Europe PMC] [Abstract]
  15. "The apoptosis-associated protein BNIPL interacts with two cell proliferation-related proteins, MIF and GFER."FEBS Lett. 540:86-90(2003) [PubMed] [Europe PMC] [Abstract]
  16. "MIF signal transduction initiated by binding to CD74."J. Exp. Med. 197:1467-1476(2003) [PubMed] [Europe PMC] [Abstract]
  17. "Macrophage migration inhibitory factor reduces the growth of virulent Mycobacterium tuberculosis in human macrophages."Infect. Immun. 73:3783-3786(2005) [PubMed] [Europe PMC] [Abstract]
  18. "Association between high levels of blood macrophage migration inhibitory factor, inappropriate adrenal response, and early death in patients with severe sepsis."Clin. Infect. Dis. 44:1321-1328(2007) [PubMed] [Europe PMC] [Abstract]
  19. "The Golgi-associated protein p115 mediates the secretion of macrophage migration inhibitory factor."J. Immunol. 182:6896-6906(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  22. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  23. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  24. "Crystal structure of macrophage migration inhibitory factor from human lymphocyte at 2.1-A resolution."FEBS Lett. 389:145-148(1996) [PubMed] [Europe PMC] [Abstract]
  25. "The crystal structure of human glycosylation-inhibiting factor is a trimeric barrel with three 6-stranded beta-sheets."Proc. Natl. Acad. Sci. U.S.A. 93:3007-3010(1996) [PubMed] [Europe PMC] [Abstract]
  26. "Crystal structure at 2.6-A resolution of human macrophage migration inhibitory factor."Proc. Natl. Acad. Sci. U.S.A. 93:5191-5196(1996) [PubMed] [Europe PMC] [Abstract]
  27. "Pro-1 of macrophage migration inhibitory factor functions as a catalytic base in the phenylpyruvate tautomerase activity."Biochemistry 38:7346-7354(1999) [PubMed] [Europe PMC] [Abstract]
  28. "Coumarin and chromen-4-one analogues as tautomerase inhibitors of macrophage migration inhibitory factor: discovery and X-ray crystallography."J. Med. Chem. 44:540-547(2001) [PubMed] [Europe PMC] [Abstract]
  29. "Alternative chemical modifications reverse the binding orientation of a pharmacophore scaffold in the active site of macrophage migration inhibitory factor."J. Biol. Chem. 282:23089-23095(2007) [PubMed] [Europe PMC] [Abstract]
  30. "Structural and kinetic analyses of macrophage migration inhibitory factor active site interactions."Biochemistry 48:132-139(2009) [PubMed] [Europe PMC] [Abstract]
  31. "MIF intersubunit disulfide mutant antagonist supports activation of CD74 by endogenous MIF trimer at physiologic concentrations."Proc. Natl. Acad. Sci. U.S.A. 110:10994-10999(2013) [PubMed] [Europe PMC] [Abstract]