Keratin 18 (KRT18)

[Edit]

CK18; CYK18; K18; Cytokeratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18

Keratin 18 (KRT18)

Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Human keratin 18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Endo B and its coexpressed type II keratin, Endo A (the mouse equivalent of human keratin 8), appear to be the first IF proteins expressed during murine development.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPB231Hu01Recombinant Keratin 18 (KRT18)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAB231Hu01Polyclonal Antibody to Keratin 18 (KRT18)WB, ICC, IHC-P, IHC-F, ELISA
MAB231Hu22Monoclonal Antibody to Keratin 18 (KRT18)WB, ICC, IHC-P, IHC-F, ELISA
LAB231Hu71Biotin-Linked Polyclonal Antibody to Keratin 18 (KRT18)IHC;WB;ELISA
LAB231Hu81FITC-Linked Polyclonal Antibody to Keratin 18 (KRT18)IHC;ICC;IF;WB
MAB231Hu21Monoclonal Antibody to Keratin 18 (KRT18)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEB231HuELISA Kit for Keratin 18 (KRT18)Enzyme-linked immunosorbent assay

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPB231Mu01Recombinant Keratin 18 (KRT18)SDS-PAGE; WB; ELISA; IP.
RPB231Mu02Recombinant Keratin 18 (KRT18)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAB231Mu01Polyclonal Antibody to Keratin 18 (KRT18)WB, ICC, IHC-P, IHC-F, ELISA
LAB231Mu71Biotin-Linked Polyclonal Antibody to Keratin 18 (KRT18)IHC;WB;ELISA
PAB231Mu02Polyclonal Antibody to Keratin 18 (KRT18)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEB231MuELISA Kit for Keratin 18 (KRT18)Enzyme-linked immunosorbent assay

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPB231Ra01Recombinant Keratin 18 (KRT18)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAB231Ra01Polyclonal Antibody to Keratin 18 (KRT18)WB, ICC, IHC-P, IHC-F, ELISA
MAB231Ra21Monoclonal Antibody to Keratin 18 (KRT18)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEB231RaELISA Kit for Keratin 18 (KRT18)Enzyme-linked immunosorbent assay

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPB231Po01Recombinant Keratin 18 (KRT18)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAB231Po01Polyclonal Antibody to Keratin 18 (KRT18)WB, ICC, IHC-P, IHC-F, ELISA
MAB231Po21Monoclonal Antibody to Keratin 18 (KRT18)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEB231PoELISA Kit for Keratin 18 (KRT18)Enzyme-linked immunosorbent assay
  1. "Comparison of mouse and human keratin 18: a component of intermediate filaments expressed prior to implantation."Differentiation 33:61-68(1986) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells."Mol. Cell. Biol. 8:1540-1550(1988) [PubMed] [Europe PMC] [Abstract]
  4. "Cytokeratin expression in simple epithelia. III. Detection of mRNAs encoding human cytokeratins nos. 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ."Differentiation 33:69-85(1986) [PubMed] [Europe PMC] [Abstract]
  5. "Cytokeratin expression in simple epithelia. I. Identification of mRNA coding for human cytokeratin no. 18 by a cDNA clone."Differentiation 30:244-253(1986) [PubMed] [Europe PMC] [Abstract]
  6. "A two-dimensional gel database of human colon carcinoma proteins."Electrophoresis 18:605-613(1997) [PubMed] [Europe PMC] [Abstract]
  7. "Characterization and dynamics of O-linked glycosylation of human cytokeratin 8 and 18."J. Biol. Chem. 267:3901-3906(1992) [PubMed] [Europe PMC] [Abstract]
  8. "Identification of the major physiologic phosphorylation site of human keratin 18: potential kinases and a role in filament reorganization."J. Cell Biol. 127:161-171(1994) [PubMed] [Europe PMC] [Abstract]
  9. "Identification and mutational analysis of the glycosylation sites of human keratin 18."J. Biol. Chem. 270:11820-11827(1995) [PubMed] [Europe PMC] [Abstract]
  10. "Chronic hepatitis, hepatocyte fragility, and increased soluble phosphoglycokeratins in transgenic mice expressing a keratin 18 conserved arginine mutant."J. Cell Biol. 131:1303-1314(1995) [PubMed] [Europe PMC] [Abstract]
  11. "14-3-3 proteins associate with phosphorylated simple epithelial keratins during cell cycle progression and act as a solubility cofactor."J. Cell Biol. 133:345-357(1996) [PubMed] [Europe PMC] [Abstract]
  12. "Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis."J. Cell Biol. 138:1379-1394(1997) [PubMed] [Europe PMC] [Abstract]
  13. "Phosphorylation of human keratin 18 serine 33 regulates binding to 14-3-3 proteins."EMBO J. 17:1892-1906(1998) [PubMed] [Europe PMC] [Abstract]
  14. "Dissection of protein linkage between keratins and pinin, a protein with dual location at desmosome-intermediate filament complex and in the nucleus."J. Biol. Chem. 275:14910-14915(2000) [PubMed] [Europe PMC] [Abstract]
  15. "Identification of Mrj, a DnaJ/Hsp40 family protein, as a keratin 8/18 filament regulatory protein."J. Biol. Chem. 275:34521-34527(2000) [PubMed] [Europe PMC] [Abstract]
  16. "Keratin attenuates tumor necrosis factor-induced cytotoxicity through association with TRADD."J. Cell Biol. 155:415-426(2001) [PubMed] [Europe PMC] [Abstract]
  17. "Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability."Biochemistry 43:1283-1295(2004) [PubMed] [Europe PMC] [Abstract]
  18. "Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease."Hepatology 40:459-466(2004) [PubMed] [Europe PMC] [Abstract]
  19. "Global proteomic approach unmasks involvement of keratins 8 and 18 in the delivery of cystic fibrosis transmembrane conductance regulator (CFTR)/deltaF508-CFTR to the plasma membrane."Proteomics 4:3833-3844(2004) [PubMed] [Europe PMC] [Abstract]
  20. "Identification of trichoplein, a novel keratin filament-binding protein."J. Cell Sci. 118:1081-1090(2005) [PubMed] [Europe PMC] [Abstract]
  21. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  22. "Rescue of DeltaF508-CFTR (cystic fibrosis transmembrane conductance regulator) by curcumin: involvement of the keratin 18 network."J. Pharmacol. Exp. Ther. 317:500-505(2006) [PubMed] [Europe PMC] [Abstract]
  23. "Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations."J. Biol. Chem. 282:8219-8227(2007) [PubMed] [Europe PMC] [Abstract]
  24. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  25. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  26. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  27. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  28. "O-GlcNAcylation determines the solubility, filament organization, and stability of keratins 8 and 18."J. Biol. Chem. 285:34062-34071(2010) [PubMed] [Europe PMC] [Abstract]
  29. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  30. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  31. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  32. "Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization."Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract]
  33. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  34. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  35. "Mutation of human keratin 18 in association with cryptogenic cirrhosis."J. Clin. Invest. 99:19-23(1997) [PubMed] [Europe PMC] [Abstract]
  36. "Keratin 8 and 18 mutations are risk factors for developing liver disease of multiple etiologies."Proc. Natl. Acad. Sci. U.S.A. 100:6063-6068(2003) [PubMed] [Europe PMC] [Abstract]