Dihydropyrimidine Dehydrogenase (DPYD)

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DPD; DHPDHase; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase

Dihydropyrimidine Dehydrogenase (DPYD)
Dihydropyrimidine dehydrogenase (DPD) is an enzyme that is involved in pyrimidine degradation. It is the initial and rate-limiting step in pyrimidine catabolism. It catalyzes the reduction of uracil and thymine. It is also involved in the degradation of the chemotherapeutic drugs 5-fluorouracil and Tegafur-uracil.The sequence of the gene suggested that DPD has at least 3 distinct domains: a possible NADPH binding site and FAD-binding site in the N terminus, 2 motifs of putative iron/sulfur-binding sites near the C terminus, and a peptide domain corresponding to the uracil-binding site.
Expression of the pig enzyme in E. coli catalyzed the reduction of uracil, thymine, and 5-fluorouracil (5FU) with kinetics approximating those published for the enzyme purified from mammalian liver.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPC012Hu01Recombinant Dihydropyrimidine Dehydrogenase (DPYD)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAC012Hu01Polyclonal Antibody to Dihydropyrimidine Dehydrogenase (DPYD)WB, ICC, IHC-P, IHC-F, ELISA
MAC012Hu22Monoclonal Antibody to Dihydropyrimidine Dehydrogenase (DPYD)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEC012HuELISA Kit for Dihydropyrimidine Dehydrogenase (DPYD)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPC012Mu01Recombinant Dihydropyrimidine Dehydrogenase (DPYD)SDS-PAGE; WB; ELISA; IP.
RPC012Mu02Recombinant Dihydropyrimidine Dehydrogenase (DPYD)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAC012Mu01Polyclonal Antibody to Dihydropyrimidine Dehydrogenase (DPYD)WB, ICC, IHC-P, IHC-F, ELISA
PAC012Mu02Polyclonal Antibody to Dihydropyrimidine Dehydrogenase (DPYD)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEC012MuELISA Kit for Dihydropyrimidine Dehydrogenase (DPYD)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Dihydropyrimidine Dehydrogenase (DPYD)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Dihydropyrimidine Dehydrogenase (DPYD)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Dihydropyrimidine Dehydrogenase (DPYD)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Dihydropyrimidine Dehydrogenase (DPYD)CLIA Kit Customized Service Offer
n/aELISA Kit for Dihydropyrimidine Dehydrogenase (DPYD)ELISA Kit Customized Service Offer
  1. "cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria."J. Biol. Chem. 269:23192-23196(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Structural organization of the human dihydropyrimidine dehydrogenase gene."Cancer Res. 57:1660-1663(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Suicidal inactivation of human dihydropyrimidine dehydrogenase by (E)-5-(2-bromovinyl)uracil derived from the antiviral, sorivudine."Cancer Lett. 122:107-113(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "A point mutation in an invariant splice donor site leads to exon skipping in two unrelated Dutch patients with dihydropyrimidine dehydrogenase deficiency."J. Inherit. Metab. Dis. 19:645-654(1996) [PubMed] [Europe PMC] [Abstract]
  8. "Lack of correlation between phenotype and genotype for the polymorphically expressed dihydropyrimidine dehydrogenase in a family of Pakistani origin."Pharmacogenetics 7:161-163(1997) [PubMed] [Europe PMC] [Abstract]
  9. "Purification and characterization of dihydropyrimidine dehydrogenase from human liver."J. Biol. Chem. 267:17102-17109(1992) [PubMed] [Europe PMC] [Abstract]
  10. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  11. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  13. "Dihydropyrimidine dehydrogenase (DPD) deficiency: identification and expression of missense mutations C29R, R886H and R235W."Hum. Genet. 101:333-338(1997) [PubMed] [Europe PMC] [Abstract]
  14. "Identification of novel point mutations in the dihydropyrimidine dehydrogenase gene."J. Inherit. Metab. Dis. 20:335-338(1997) [PubMed] [Europe PMC] [Abstract]
  15. "DNA sequencing of a cytogenetically normal acute myeloid leukaemia genome." Nature 456:66-72(2008) [PubMed] [Europe PMC] [Abstract]