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Deoxycytidine Kinase (DCK)

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Deoxycytidine Kinase (DCK)
CCC C440
HGNC 2704
MGI 102726
PubMed 1406603
RGD 620667
UniProt P27707
Wiki DCK

Deoxycytidine kinase (DCK) is responsible for the phosphorylation of several deoxyribonucleosides and their analogs. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. It is clinically important because of its relationship to drug resistance and sensitivity. It is the rate limiting enzyme in the activation of many important anticancer and retroviral drugs and its activity is often decreased in cells that are resistant to cytosine arabinoside.Human deoxyribonucleoside kinases are required for the pharmacologic activity of several clinically important anticancer and antiviral nucleoside analogs.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC440Hu01 Recombinant Deoxycytidine Kinase (DCK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC440Hu01 Polyclonal Antibody to Deoxycytidine Kinase (DCK) WB; IHC; ICC; IP.
MAC440Hu22 Monoclonal Antibody to Deoxycytidine Kinase (DCK) WB; IHC; ICC; IP.
Assay Kits SEC440Hu ELISA Kit for Deoxycytidine Kinase (DCK) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxycytidine Kinase (DCK) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxycytidine Kinase (DCK) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxycytidine Kinase (DCK) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxycytidine Kinase (DCK) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxycytidine Kinase (DCK) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxycytidine Kinase (DCK) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxycytidine Kinase (DCK) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxycytidine Kinase (DCK) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxycytidine Kinase (DCK) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxycytidine Kinase (DCK) ELISA Kit Customized Service Offer
  1. "Cloning and expression of human deoxycytidine kinase cDNA."Proc. Natl. Acad. Sci. U.S.A. 88:1531-1535(1991) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Characterization of human deoxycytidine kinase. Correlation with cDNA sequences."FEBS Lett. 280:363-366(1991) [PubMed] [Europe PMC] [Abstract]
  5. "Human deoxycytidine kinase is located in the cell nucleus."Proc. Natl. Acad. Sci. U.S.A. 94:11941-11945(1997) [PubMed] [Europe PMC] [Abstract]
  6. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  7. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Casein kinase 1delta activates human recombinant deoxycytidine kinase by Ser-74 phosphorylation, but is not involved in the in vivo regulation of its activity."Arch. Biochem. Biophys. 502:44-52(2010) [PubMed] [Europe PMC] [Abstract]
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  13. "Structure of human dCK suggests strategies to improve anticancer and antiviral therapy."Nat. Struct. Biol. 10:513-519(2003) [PubMed] [Europe PMC] [Abstract]
  14. "The structure of human deoxycytidine kinase in complex with clofarabine reveals key interactions for prodrug activation."Acta Crystallogr. D 62:133-139(2006) [PubMed] [Europe PMC] [Abstract]
  15. "Nonenantioselectivity property of human deoxycytidine kinase explained by structures of the enzyme in complex with L- and D-nucleosides."J. Med. Chem. 50:3004-3014(2007) [PubMed] [Europe PMC] [Abstract]
  16. "Structural basis for substrate promiscuity of dCK."J. Mol. Biol. 378:607-621(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Extending thymidine kinase activity to the catalytic repertoire of human deoxycytidine kinase."Biochemistry 48:1256-1263(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Lead optimization and structure-based design of potent and bioavailable deoxycytidine kinase inhibitors." Bioorg. Med. Chem. Lett. 19:6784-6787(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Structural and kinetic characterization of human deoxycytidine kinase variants able to phosphorylate 5-substituted deoxycytidine and thymidine analogues."Biochemistry 49:6784-6790(2010) [PubMed] [Europe PMC] [Abstract]
  20. "The sugar ring of the nucleoside is required for productive substrate positioning in the active site of human deoxycytidine kinase (dCK): implications for the development of dCK-activated acyclic guanine analogues."J. Med. Chem. 53:5792-5800(2010) [PubMed] [Europe PMC] [Abstract]