≡

Growth Factor Receptor Bound Protein 2 (Grb2)

[Edit]

ASH; NCKAP2; EGFRBP-GRB2; Grb3-3; Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2

Growth Factor Receptor Bound Protein 2 (Grb2)
CCC C514
HGNC 4566
MGI 95805
PubMed 18620523
RGD 619758
UniProt P62993
Wiki Grb2
Growth factor receptor-bound protein 2 also known as Grb2 is an adaptor protein involved in signal transduction/cell communication. In humans, the GRB2 protein is encoded by the GRB2 gene.
The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC514Hu01 Recombinant Growth Factor Receptor Bound Protein 2 (Grb2) Positive Control; Immunogen; SDS-PAGE; WB.
EPC514Hu61 Eukaryotic Growth Factor Receptor Bound Protein 2 (Grb2) Positive Control; Immunogen; SDS-PAGE; WB.
APC514Hu61 Active Growth Factor Receptor Bound Protein 2 (Grb2) Cell culture; Activity Assays.
Antibodies PAC514Hu01 Polyclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2) WB; IHC; ICC; IP.
MAC514Hu22 Monoclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2) WB; IHC; ICC; IP.
PAC514Hu06 Polyclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2) WB; IHC; ICC; IP.
Assay Kits SEC514Hu ELISA Kit for Growth Factor Receptor Bound Protein 2 (Grb2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Growth Factor Receptor Bound Protein 2 (Grb2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2) Polyclonal Antibody Customized Service Offer
Assay Kits SEC514Mu ELISA Kit for Growth Factor Receptor Bound Protein 2 (Grb2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Growth Factor Receptor Bound Protein 2 (Grb2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Growth Factor Receptor Bound Protein 2 (Grb2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Growth Factor Receptor Bound Protein 2 (Grb2) ELISA Kit Customized Service Offer
  1. "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling."Cell 70:431-442(1992) [PubMed] [Europe PMC] [Abstract]
  2. "Cloning of ASH, a ubiquitous protein composed of one Src homology region (SH) 2 and two SH3 domains, from human and rat cDNA libraries."Proc. Natl. Acad. Sci. U.S.A. 89:9015-9019(1992) [PubMed] [Europe PMC] [Abstract]
  3. "Cloning of a Grb2 isoform with apoptotic properties."Science 264:971-974(1994) [PubMed] [Europe PMC] [Abstract]
  4. "The gene structure of the human growth factor bound protein GRB2."Genomics 56:203-207(1999) [PubMed] [Europe PMC] [Abstract]
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. , Submitted (DEC-2008) to UniProtKB
  8. "Mutation of Tyr697, a GRB2-binding site, and Tyr721, a PI 3-kinase binding site, abrogates signal transduction by the murine CSF-1 receptor expressed in Rat-2 fibroblasts."EMBO J. 12:5161-5172(1993) [PubMed] [Europe PMC] [Abstract]
  9. "Insulin stimulates association of insulin receptor substrate-1 with the protein abundant Src homology/growth factor receptor-bound protein 2."J. Biol. Chem. 268:11167-11171(1993) [PubMed] [Europe PMC] [Abstract]
  10. "The SH2/SH3 domain-containing protein GRB2 interacts with tyrosine-phosphorylated IRS1 and Shc: implications for insulin control of ras signalling."EMBO J. 12:1929-1936(1993) [PubMed] [Europe PMC] [Abstract]
  11. "Association of the DF3/MUC1 breast cancer antigen with Grb2 and the Sos/Ras exchange protein."Cancer Res. 55:4000-4003(1995) [PubMed] [Europe PMC] [Abstract]
  12. "Multiple forms of an inositol polyphosphate 5-phosphatase form signaling complexes with Shc and Grb2."Curr. Biol. 6:438-445(1996) [PubMed] [Europe PMC] [Abstract]
  13. "Ligand activation of ELK receptor tyrosine kinase promotes its association with Grb10 and Grb2 in vascular endothelial cells."J. Biol. Chem. 271:23588-23593(1996) [PubMed] [Europe PMC] [Abstract]
  14. "Purification and molecular cloning of SH2- and SH3-containing inositol polyphosphate-5-phosphatase, which is involved in the signaling pathway of granulocyte-macrophage colony-stimulating factor, erythropoietin, and Bcr-Abl."Blood 89:2745-2756(1997) [PubMed] [Europe PMC] [Abstract]
  15. "Focal adhesion kinase overexpression enhances ras-dependent integrin signaling to ERK2/mitogen-activated protein kinase through interactions with and activation of c-Src."J. Biol. Chem. 272:13189-13195(1997) [PubMed] [Europe PMC] [Abstract]
  16. "A family of proteins that inhibit signalling through tyrosine kinase receptors."Nature 386:181-186(1997) [PubMed] [Europe PMC] [Abstract]
  17. "Intracellular signaling of the Ufo/Axl receptor tyrosine kinase is mediated mainly by a multi-substrate docking-site."Oncogene 14:2619-2631(1997) [PubMed] [Europe PMC] [Abstract]
  18. "Cloning and characterization of APS, an adaptor molecule containing PH and SH2 domains that is tyrosine phosphorylated upon B-cell receptor stimulation."Oncogene 15:7-15(1997) [PubMed] [Europe PMC] [Abstract]
  19. "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation."Cell 92:83-92(1998) [PubMed] [Europe PMC] [Abstract]
  20. "Identification and characterization of a novel protein interacting with Ral-binding protein 1, a putative effector protein of Ral."J. Biol. Chem. 273:814-821(1998) [PubMed] [Europe PMC] [Abstract]
  21. "Characterization of insulin receptor substrate 4 in human embryonic kidney 293 cells."J. Biol. Chem. 273:10726-10732(1998) [PubMed] [Europe PMC] [Abstract]
  22. "Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins."Oncogene 16:891-901(1998) [PubMed] [Europe PMC] [Abstract]
  23. "Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck."J. Biol. Chem. 274:5542-5549(1999) [PubMed] [Europe PMC] [Abstract]
  24. "Tyrosine phosphorylation and complex formation of Cbl-b upon T cell receptor stimulation."Oncogene 18:1147-1156(1999) [PubMed] [Europe PMC] [Abstract]
  25. "cbl-b inhibits epidermal growth factor receptor signaling."Oncogene 18:1855-1866(1999) [PubMed] [Europe PMC] [Abstract]
  26. "NS5A, a nonstructural protein of hepatitis C virus, binds growth factor receptor-bound protein 2 adaptor protein in a Src homology 3 domain/ligand-dependent manner and perturbs mitogenic signaling."Proc. Natl. Acad. Sci. U.S.A. 96:5533-5538(1999) [PubMed] [Europe PMC] [Abstract]
  27. "Identification and characterization of a new family of guanine nucleotide exchange factors for the ras-related GTPase Ral."J. Biol. Chem. 275:13406-13410(2000) [PubMed] [Europe PMC] [Abstract]
  28. "Ligand discrimination in signaling through an ErbB4 receptor homodimer."J. Biol. Chem. 275:19803-19807(2000) [PubMed] [Europe PMC] [Abstract]
  29. "Interaction of linker for activation of T cells with multiple adapter proteins in platelets activated by the glycoprotein VI-selective ligand, convulxin."J. Biol. Chem. 275:33427-33434(2000) [PubMed] [Europe PMC] [Abstract]
  30. "Generation of novel cytoplasmic forms of protein tyrosine phosphatase epsilon by proteolytic processing and translational control."Oncogene 19:4375-4384(2000) [PubMed] [Europe PMC] [Abstract]
  31. "Structural and functional dissection of the cytoplasmic domain of the transmembrane adaptor protein SIT (SHP2-interacting transmembrane adaptor protein)."Eur. J. Immunol. 31:1825-1836(2001) [PubMed] [Europe PMC] [Abstract]
  32. "The ORF3 protein of hepatitis E virus binds to Src homology 3 domains and activates MAPK."J. Biol. Chem. 276:42389-42400(2001) [PubMed] [Europe PMC] [Abstract]
  33. "Recruitment of the class II phosphoinositide 3-kinase C2beta to the epidermal growth factor receptor: role of Grb2."Mol. Cell. Biol. 21:6660-6667(2001) [PubMed] [Europe PMC] [Abstract]
  34. "Insulin receptor substrate 4 associates with the protein IRAS."J. Biol. Chem. 277:19439-19447(2002) [PubMed] [Europe PMC] [Abstract]
  35. "SKAP55 recruits to lipid rafts and positively mediates the MAPK pathway upon T cell receptor activation."J. Biol. Chem. 277:40420-40427(2002) [PubMed] [Europe PMC] [Abstract]
  36. "Molecular cloning of a novel gene encoding a membrane-associated adaptor protein (LAX) in lymphocyte signaling."J. Biol. Chem. 277:46151-46158(2002) [PubMed] [Europe PMC] [Abstract]
  37. "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling."J. Exp. Med. 196:1617-1626(2002) [PubMed] [Europe PMC] [Abstract]
  38. "Hepatocyte growth factor receptor tyrosine kinase met is a substrate of the receptor protein-tyrosine phosphatase DEP-1."J. Biol. Chem. 278:5728-5735(2003) [PubMed] [Europe PMC] [Abstract]
  39. "EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis."J. Cell Biol. 162:661-671(2003) [PubMed] [Europe PMC] [Abstract]
  40. "LAB: a new membrane-associated adaptor molecule in B cell activation."Nat. Immunol. 4:117-123(2003) [PubMed] [Europe PMC] [Abstract]
  41. "TrkA alternative splicing: a regulated tumor-promoting switch in human neuroblastoma."Cancer Cell 6:347-360(2004) [PubMed] [Europe PMC] [Abstract]
  42. "Activation of vascular endothelial growth factor receptor-3 and its downstream signaling promote cell survival under oxidative stress."J. Biol. Chem. 279:27088-27097(2004) [PubMed] [Europe PMC] [Abstract]
  43. "Signal transduction via the stem cell factor receptor/c-Kit."Cell. Mol. Life Sci. 61:2535-2548(2004) [PubMed] [Europe PMC] [Abstract]
  44. "The c-Fes tyrosine kinase cooperates with the breakpoint cluster region protein (Bcr) to induce neurite extension in a Rac- and Cdc42-dependent manner."Exp. Cell Res. 299:188-198(2004) [PubMed] [Europe PMC] [Abstract]
  45. "Signaling by Kit protein-tyrosine kinase--the stem cell factor receptor."Biochem. Biophys. Res. Commun. 337:1-13(2005) [PubMed] [Europe PMC] [Abstract]
  46. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  47. "NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells."Nat. Immunol. 7:524-532(2006) [PubMed] [Europe PMC] [Abstract]
  48. "Identification of a new transmembrane adaptor protein that constitutively binds Grb2 in B cells."J. Leukoc. Biol. 84:842-851(2008) [PubMed] [Europe PMC] [Abstract]
  49. "Distinct functions of natural ADAM-15 cytoplasmic domain variants in human mammary carcinoma."Mol. Cancer Res. 6:383-394(2008) [PubMed] [Europe PMC] [Abstract]
  50. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  51. "An unbiased screen identifies DEP-1 tumor suppressor as a phosphatase controlling EGFR endocytosis."Curr. Biol. 19:1788-1798(2009) [PubMed] [Europe PMC] [Abstract]
  52. "Cytoplasmic ACK1 interaction with multiple receptor tyrosine kinases is mediated by Grb2: an analysis of ACK1 effects on Axl signaling."J. Biol. Chem. 284:34954-34963(2009) [PubMed] [Europe PMC] [Abstract]
  53. "KIF26A is an unconventional kinesin and regulates GDNF-Ret signaling in enteric neuronal development."Cell 139:802-813(2009) [PubMed] [Europe PMC] [Abstract]
  54. "GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through the activation of extracellular signal-regulated kinase signaling."J. Biol. Chem. 284:20206-20214(2009) [PubMed] [Europe PMC] [Abstract]
  55. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  56. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  57. "Recruitment of Pyk2 to SHPS-1 signaling complex is required for IGF-I-dependent mitogenic signaling in vascular smooth muscle cells."Cell. Mol. Life Sci. 67:3893-3903(2010) [PubMed] [Europe PMC] [Abstract]
  58. "Histidine domain-protein tyrosine phosphatase interacts with Grb2 and GrpL."PLoS ONE 5:E14339-E14339(2010) [PubMed] [Europe PMC] [Abstract]
  59. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  60. "ACAP4 protein cooperates with Grb2 protein to orchestrate epidermal growth factor-stimulated integrin beta1 recycling in cell migration."J. Biol. Chem. 286:43735-43747(2011) [PubMed] [Europe PMC] [Abstract]
  61. "SCIMP, a transmembrane adapter protein involved in major histocompatibility complex class II signaling."Mol. Cell. Biol. 31:4550-4562(2011) [PubMed] [Europe PMC] [Abstract]
  62. "Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling."Nat. Immunol. 13:560-568(2012) [PubMed] [Europe PMC] [Abstract]
  63. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  64. "Role of herpes simplex virus VP11/12 tyrosine-based motifs in binding and activation of the Src family kinase Lck and recruitment of p85, Grb2, and Shc."J. Virol. 87:11276-11286(2013) [PubMed] [Europe PMC] [Abstract]
  65. "Nuclear magnetic resonance solution structure of the growth factor receptor-bound protein 2 Src homology 2 domain."Biochemistry 35:11852-11864(1996) [PubMed] [Europe PMC] [Abstract]
  66. "The three-dimensional solution structure of the Src homology domain-2 of the growth factor receptor-bound protein-2."J. Biomol. NMR 11:153-164(1998)
  67. "Solution structure and ligand-binding site of the carboxy-terminal SH3 domain of GRB2."Structure 2:1029-1040(1994) [PubMed] [Europe PMC] [Abstract]
  68. "Crystal structure of the mammalian Grb2 adaptor."Science 268:291-293(1995) [PubMed] [Europe PMC] [Abstract]
  69. "Structural basis for the high affinity of amino-aromatic SH2 phosphopeptide ligands."J. Mol. Biol. 279:1013-1022(1998) [PubMed] [Europe PMC] [Abstract]
  70. "Structural and conformational requirements for high-affinity binding to the SH2 domain of Grb2(1)."J. Med. Chem. 42:971-980(1999) [PubMed] [Europe PMC] [Abstract]
  71. "Structure-based design, synthesis, and X-ray crystallography of a high- affinity antagonist of the Grb2-SH2 domain containing an asparagine mimetic."J. Med. Chem. 42:2358-2363(1999) [PubMed] [Europe PMC] [Abstract]
  72. "Dimer formation through domain swapping in the crystal structure of the Grb2-SH2-Ac-pYVNV complex."Biochemistry 39:13376-13382(2000) [PubMed] [Europe PMC] [Abstract]
  73. "Crystal structures of the SH2 domain of Grb2: highlight on the binding of a new high-affinity inhibitor."J. Mol. Biol. 315:1167-1177(2002) [PubMed] [Europe PMC] [Abstract]
  74. "Distinct binding modes of two epitopes in Gab2 that interact with the SH3C domain of Grb2."Structure 17:809-822(2009) [PubMed] [Europe PMC] [Abstract]
  75. "High resolution crystal structure of the Grb2 SH2 domain with a phosphopeptide derived from CD28."PLoS ONE 8:E74482-E74482(2013) [PubMed] [Europe PMC] [Abstract]