≡

Legumain (LGMN)

[Edit]

AEP; LGMN1; PRSC1; Protease,Cysteine 1; Asparaginyl endopeptidase

Legumain (LGMN)
CCC C564
HGNC 9472
MGI 1330838
PubMed 22441772
RGD 619832
UniProt Q99538
Wiki LGMN
Legumain is a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC564Hu01 Recombinant Legumain (LGMN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC564Hu01 Polyclonal Antibody to Legumain (LGMN) WB; IHC; ICC; IP.
MAC564Hu22 Monoclonal Antibody to Legumain (LGMN) WB; IHC; ICC; IP.
Assay Kits SEC564Hu ELISA Kit for Legumain (LGMN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC564Mu01 Recombinant Legumain (LGMN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC564Mu02 Recombinant Legumain (LGMN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC564Mu01 Polyclonal Antibody to Legumain (LGMN) WB; IHC; ICC; IP.
PAC564Mu02 Polyclonal Antibody to Legumain (LGMN) WB; IHC; ICC; IP.
Assay Kits SEC564Mu ELISA Kit for Legumain (LGMN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Legumain (LGMN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Legumain (LGMN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Legumain (LGMN) Polyclonal Antibody Customized Service Offer
Assay Kits SEC564Ra ELISA Kit for Legumain (LGMN) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase."J. Biol. Chem. 272:8090-8098(1997) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning of a human cDNA encoding putative cysteine protease (PRSC1) and its chromosome assignment to 14q32.1."Cytogenet. Cell Genet. 74:120-123(1996) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and analysis of human chromosome 14." Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Activation of human prolegumain by cleavage at a C-terminal asparagine residue."Biochem. J. 352:327-334(2000) [PubMed] [Europe PMC] [Abstract]
  6. "Autocatalytic activation of human legumain at aspartic acid residues."FEBS Lett. 438:114-118(1998) [PubMed] [Europe PMC] [Abstract]
  7. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  8. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  10. "Mechanistic and structural studies on legumain explain its zymogenicity, distinct activation pathways, and regulation."Proc. Natl. Acad. Sci. U.S.A. 110:10940-10945(2013) [PubMed] [Europe PMC] [Abstract]