Chloramphenicol Acetyltransferase (CAT)

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Chloramphenicol Acetyltransferase (CAT)
Acetyltransferase (or transacetylase) is a type of transferase enzyme which transfers an acetyl group.include:Histone acetyltransferases including CBP histone acetyltransferase ; Choline acetyltransferase ; Chloramphenicol acetyltransferase; Serotonin N-acetyl transferase .Chloramphenicol acetyltransferase (or CAT) is a bacterial enzyme that detoxifies the antibiotic chloramphenicol and is responsible for chloramphenicol resistance in bacteria.
This enzyme covalently attaches an acetyl group from acetyl-CoA to chloramphenicol, which prevents chloramphenicol from binding to ribosomes.CAT is used as a reporter system to measure the level of a promoter or its tissue-specific expression. The CAT assay involves monitoring acetylation of radioactive chloramphenicol on a TCA plate because acetylation significantly changes migration rate.

Organism species: Escherichia coli

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Chloramphenicol Acetyltransferase (CAT)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Chloramphenicol Acetyltransferase (CAT)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Chloramphenicol Acetyltransferase (CAT)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Chloramphenicol Acetyltransferase (CAT)CLIA Kit Customized Service Offer
n/aELISA Kit for Chloramphenicol Acetyltransferase (CAT)ELISA Kit Customized Service Offer
  1. "Primary structure of a chloramphenicol acetyltransferase specified by R plasmids."Nature 282:870-872(1979) [PubMed] [Europe PMC] [Abstract]
  2. "Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9."Nature 282:864-869(1979) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence of an IS/-flanked transposon coding for resistance to chloramphenicol and fusidic acid."FEBS Lett. 110:11-14(1980) [PubMed] [Europe PMC] [Abstract]
  4. "The structure of nitric oxide synthase oxygenase domain and inhibitor complexes."Science 278:425-431(1997) [PubMed] [Europe PMC] [Abstract]