Phospholipase C Delta 4 (PLCd4)

The purified PLCD4 protein exhibited an apparent molecular mass of 90 kD. The distribution of PLCD4 in 16 different rat tissues was studied by immunoblot analysis with PLCD4-specific antibodies of fractions obtained after an enzyme-enrichment procedure. The 90-kD immunoreactive protein was detected unambiguously in only 8 tissues and was present at concentrations that were low compared to those of other major PLC isozymes. An 86-kD band was detectable in spleen. A 93-kD immunoreactive protein was also prominent in testis but was not detected in the other 7 positive tissues. The 93-kD enzyme appears to be derived from a splice variant of the mRNA that encodes the 90-kD PLCD4 and contains an additional 32 amino acids between the X and Y catalytic domains.