Puromycin Sensitive Aminopeptidase (PSA)

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NPEPPS; AAP-S; Metalloproteinase MP100; Cytosol alanyl aminopeptidase

Puromycin Sensitive Aminopeptidase (PSA)
This puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle.
PSA contains a zinc-binding motif conserved among gluzincin aminopeptidases and shares 98% sequence identity with the mouse protein. Northern blot analysis detected ubiquitous expression of a 4.8-kb transcript, with highest expression in brain. By in situ hybridization of adult human brain sections, expression was localized to the perikaryon of neurons of the cortex and cerebellum.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPE206Hu01Recombinant Puromycin Sensitive Aminopeptidase (PSA)SDS-PAGE; WB; ELISA; IP.
RPE206Hu02Recombinant Puromycin Sensitive Aminopeptidase (PSA)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAE206Hu01Polyclonal Antibody to Puromycin Sensitive Aminopeptidase (PSA)WB, ICC, IHC-P, IHC-F, ELISA
PAE206Hu02Polyclonal Antibody to Puromycin Sensitive Aminopeptidase (PSA)WB, ICC, IHC-P, IHC-F, ELISA
LAE206Hu71Biotin-Linked Polyclonal Antibody to Puromycin Sensitive Aminopeptidase (PSA)IHC;WB;ELISA
Assay KitsSEE206HuELISA Kit for Puromycin Sensitive Aminopeptidase (PSA)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPE206Mu01Recombinant Puromycin Sensitive Aminopeptidase (PSA)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAE206Mu01Polyclonal Antibody to Puromycin Sensitive Aminopeptidase (PSA)WB, ICC, IHC-P, IHC-F, ELISA
LAE206Mu71Biotin-Linked Polyclonal Antibody to Puromycin Sensitive Aminopeptidase (PSA)IHC;WB;ELISA
Assay Kitsn/aCLIA Kit for Puromycin Sensitive Aminopeptidase (PSA)CLIA Kit Customized Service Offer
n/aELISA Kit for Puromycin Sensitive Aminopeptidase (PSA)ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Puromycin Sensitive Aminopeptidase (PSA)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Puromycin Sensitive Aminopeptidase (PSA)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Puromycin Sensitive Aminopeptidase (PSA)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Puromycin Sensitive Aminopeptidase (PSA)CLIA Kit Customized Service Offer
n/aELISA Kit for Puromycin Sensitive Aminopeptidase (PSA)ELISA Kit Customized Service Offer
  1. "Cloning of the human puromycin-sensitive aminopeptidase and evidence for expression in neurons."J. Neurochem. 68:889-897(1997) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Human puromycin-sensitive aminopeptidase: cloning of 3' UTR, evidence for a polymorphism at a.a. 140 and refined chromosomal localization to 17q21."Cytogenet. Cell Genet. 92:221-224(2001) [PubMed] [Europe PMC] [Abstract]
  6. "Cloning and analysis of the gene for the human puromycin-sensitive aminopeptidase."Biochem. Biophys. Res. Commun. 258:234-240(1999) [PubMed] [Europe PMC] [Abstract]
  7. "Puromycin-sensitive alanyl aminopeptidase from human liver cytosol: purification and characterization."Forensic Sci. Int. 113:143-146(2000) [PubMed] [Europe PMC] [Abstract]
  8. "Two new proteases in the MHC class I processing pathway."Nat. Immunol. 1:413-418(2000) [PubMed] [Europe PMC] [Abstract]
  9. "Mutation of active site residues of the puromycin-sensitive aminopeptidase: conversion of the enzyme into a catalytically inactive binding protein."Arch. Biochem. Biophys. 413:236-242(2003) [PubMed] [Europe PMC] [Abstract]
  10. "Degradation of tau protein by puromycin-sensitive aminopeptidase in vitro."Biochemistry 45:15111-15119(2006) [PubMed] [Europe PMC] [Abstract]
  11. "Puromycin-sensitive aminopeptidase is the major peptidase responsible for digesting polyglutamine sequences released by proteasomes during protein degradation."EMBO J. 26:1385-1396(2007) [PubMed] [Europe PMC] [Abstract]
  12. "Cytosolic aminopeptidases influence MHC class I-mediated antigen presentation in an allele-dependent manner."J. Immunol. 183:7379-7387(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]