Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)

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Ubiquitin Thiolesterase; Ubiquitin thioesterase L3; Ubiquitin carboxyl-terminal hydrolase isozyme L3

Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)
Covalent attachment of the C terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. The predicted 230-amino acid human UCHL3 protein is 54% identical to UCHL1.In adult tissues, Uchl3 was variably expressed in all tissues examined, with highest expression in testis.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPG944Hu01Recombinant Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAG944Hu01Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)WB, ICC, IHC-P, IHC-F, ELISA
LAG944Hu71Biotin-Linked Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)IHC;WB;ELISA
Assay Kitsn/aCLIA Kit for Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)CLIA Kit Customized Service Offer
n/aELISA Kit for Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)CLIA Kit Customized Service Offer
n/aELISA Kit for Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPG944Ra01Recombinant Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAG944Ra01Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)WB, ICC, IHC-P, IHC-F, ELISA
LAG944Ra71Biotin-Linked Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)IHC;WB;ELISA
Assay Kitsn/aCLIA Kit for Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)CLIA Kit Customized Service Offer
n/aELISA Kit for Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)ELISA Kit Customized Service Offer
  1. "The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase."Science 246:670-673(1989) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and analysis of human chromosome 13." Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Cleavage of the C-terminus of NEDD8 by UCH-L3."Biochem. Biophys. Res. Commun. 251:688-692(1998) [PubMed] [Europe PMC] [Abstract]
  6. "Molecular profiling of the immune response in colon cancer using protein microarrays: occurrence of autoantibodies to ubiquitin C-terminal hydrolase L3."Proteomics 3:2108-2115(2003) [PubMed] [Europe PMC] [Abstract]
  7. "Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry."Biochemistry 43:6535-6544(2004) [PubMed] [Europe PMC] [Abstract]
  8. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  9. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Ubiquitin dimers control the hydrolase activity of UCH-L3."Neurochem. Int. 54:314-321(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Skeletal muscles of Uchl3 knockout mice show polyubiquitinated protein accumulation and stress responses."Neurochem. Int. 56:911-918(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  13. "Mutant ubiquitin (UBB(+1)) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3)."FEBS Lett. 585:2568-2574(2011) [PubMed] [Europe PMC] [Abstract]
  14. "Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes."ChemBioChem 13:1416-1420(2012) [PubMed] [Europe PMC] [Abstract]
  15. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  16. "Crystal structure of a deubiquitinating enzyme (human UCH-L3) at 1.8-A resolution."EMBO J. 16:3787-3796(1997) [PubMed] [Europe PMC] [Abstract]
  17. "Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate."J. Biol. Chem. 280:1512-1520(2005) [PubMed] [Europe PMC] [Abstract]