≡

Stratifin (SFN)

[Edit]

YWHAS; HME1; 14-3-3 Protein Sigma; Epithelial cell marker protein 1

Stratifin (SFN)
CCC H179
HGNC 10773
MGI 1891831
PubMed 22310466
RGD 1304729
UniProt P31947
Wiki Stratifin
Stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Exogenous introduction of 14-3-3-sigma into cycling cells results in a G2 arrest. After DNA damage, these cells initially arrested in the G2 phase of the cell cycle, but unlike cells containing 14-3-3-sigma, the 14-3-3-sigma -/- cells were unable to maintain cell cycle arrest. The 14-3-3-sigma -/- cells died (mitotic catastrophe) as they entered mitosis. This process was associated with a failure of the 14-3-3-sigma-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH179Hu01 Recombinant Stratifin (SFN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH179Hu01 Polyclonal Antibody to Stratifin (SFN) WB
LAH179Hu71 Biotin-Linked Polyclonal Antibody to Stratifin (SFN) WB; IHC; ICC.
LAH179Hu81 FITC-Linked Polyclonal Antibody to Stratifin (SFN) WB; IHC; ICC; IF.
MAH179Hu21 Monoclonal Antibody to Stratifin (SFN) WB; IHC; ICC; IP.
Assay Kits SEH179Hu ELISA Kit for Stratifin (SFN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH179Mu01 Recombinant Stratifin (SFN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH179Mu01 Polyclonal Antibody to Stratifin (SFN) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Stratifin (SFN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Stratifin (SFN) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Stratifin (SFN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Stratifin (SFN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Stratifin (SFN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Stratifin (SFN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Stratifin (SFN) ELISA Kit Customized Service Offer
  1. "Complementary DNA cloning of a novel epithelial cell marker protein, HME1, that may be down-regulated in neoplastic mammary cells."Cell Growth Differ. 3:507-513(1992) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning and expression of the transformation sensitive epithelial marker stratifin. A member of a protein family that has been involved in the protein kinase C signalling pathway."J. Mol. Biol. 231:982-998(1993) [PubMed] [Europe PMC] [Abstract]
  3. "14-3-3 sigma is a p53-regulated inhibitor of G2/M progression."Mol. Cell 1:3-11(1997) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract]
  7. "Exportin 7 defines a novel general nuclear export pathway."EMBO J. 23:3227-3236(2004) [PubMed] [Europe PMC] [Abstract]
  8. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  9. "CARPs enhance p53 turnover by degrading 14-3-3sigma and stabilizing MDM2."Cell Cycle 7:670-682(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein."EMBO J. 27:2305-2316(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  12. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Interaction of Akt-phosphorylated SRPK2 with 14-3-3 mediates cell cycle and cell death in neurons."J. Biol. Chem. 284:24512-24525(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  15. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  16. "COP9 signalosome subunit 6 stabilizes COP1, which functions as an E3 ubiquitin ligase for 14-3-3sigma."Oncogene 30:4791-4801(2011) [PubMed] [Europe PMC] [Abstract]
  17. "A structural basis for 14-3-3sigma functional specificity."J. Biol. Chem. 280:18891-18898(2005) [PubMed] [Europe PMC] [Abstract]
  18. "Identification and structural characterization of two 14-3-3 binding sites in the human peptidylarginine deiminase type VI."J. Struct. Biol. 180:65-72(2012) [PubMed] [Europe PMC] [Abstract]