Nexilin (NEXN)


NELIN; F Actin Binding Protein

Nexilin (NEXN)
The name nexilin came from the Latin word nexilis, meaning 'bound together.' Full-length b-nexilin contains an N-terminal F-actin-binding domain (ABD), followed by a spacer, a coiled-coil region, a second ABD, and a long C-terminal tail. In comparison, s-nexilin lacks the N-terminal ABD and has an insertion in the C-terminal tail. Immunofluorescence microscopy revealed that both b-nexilin and s-nexilin colocalized with F-actin at focal contacts at the ends of stress fibers.
The nexilins did not localize to cell-cell junctions. Western blot analysis detected nexilin proteins with apparent molecular masses above 90 kD in rat brain, testis, and spleen and in cultured rodent fibroblasts. Neither protein was detected in liver, kidney, or epithelial cell lines.

Organism species: Homo sapiens (Human)

ProteinsRPH548Hu01Recombinant Nexilin (NEXN)Positive Control; Immunogen; SDS-PAGE; WB.
AntibodiesPAH548Hu01Polyclonal Antibody to Nexilin (NEXN)WB; IHC; ICC; IP.
MAH548Hu21Monoclonal Antibody to Nexilin (NEXN)WB; IHC; ICC; IP.
Assay KitsSEH548HuELISA Kit for Nexilin (NEXN)Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

ProteinsRPH548Mu01Recombinant Nexilin (NEXN)Positive Control; Immunogen; SDS-PAGE; WB.
AntibodiesPAH548Mu01Polyclonal Antibody to Nexilin (NEXN)WB; IHC; ICC; IP.
Assay Kitsn/aCLIA Kit for Nexilin (NEXN)CLIA Kit Customized Service Offer
n/aELISA Kit for Nexilin (NEXN)ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

ProteinsRPH548Ra01Recombinant Nexilin (NEXN)Positive Control; Immunogen; SDS-PAGE; WB.
AntibodiesPAH548Ra01Polyclonal Antibody to Nexilin (NEXN)WB; IHC; ICC; IP.
Assay Kitsn/aCLIA Kit for Nexilin (NEXN)CLIA Kit Customized Service Offer
n/aELISA Kit for Nexilin (NEXN)ELISA Kit Customized Service Offer
  1. "Nexilin mutations destabilize cardiac Z-disks and lead to dilated cardiomyopathy."Nat. Med. 15:1281-1288(2009) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract].
  3. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract].
  5. "Molecular cloning of NELIN, a putative human cytoskeleton regulation gene."Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 33:19-24(2001) [PubMed] [Europe PMC] [Abstract].
  6. "Muscle autoantigens in thyroid associated ophthalmopathy: the limits of molecular genetics."J. Endocrinol. Invest. 16:533-540(1993) [PubMed] [Europe PMC] [Abstract]
  7. "NELIN, a new F-actin associated protein, stimulates HeLa cell migration and adhesion."Biochem. Biophys. Res. Commun. 330:1127-1131(2005) [PubMed] [Europe PMC] [Abstract]
  8. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  9. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  10. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  15. "Mutations in NEXN, a Z-disc gene, are associated with hypertrophic cardiomyopathy."Am. J. Hum. Genet. 87:687-693(2010) [PubMed] [Europe PMC] [Abstract]