Neonatal Dermal Fibroblasts (NDF)

Dermal fibroblasts(DF) are the major cells in the dermis, which normally proliferate and differentiate to maintain the normal structure and physiological function of the skin. Due to the dermal fibroblasts with strong division and proliferation ability, strong adaptability, easy to cultivate, stable properties, and secrete some cytokines and extracellular matrix components, can promote epidermal cell proliferation, migration and maturation, in regulating the morphology of epidermal cells and dermal recombinant play an important role.Furthermore, these dermal fibroblasts produce the protein molecules including laminin and fibronectin which comprise the extracellular matrix. By creating the extracellular matrix between the dermis and epidermis, fibroblasts allow the epithelial cells of the epidermis to affix the matrix, thereby allowing the epidermal cells to effectively join together to form the top layer of the skin.Neonatal dermal fibroblasts (NDF) are isolated from neonatal animal skin.Neonatal dermal fibroblasts were evaluated as a potential cell source for intervertebral disc repair. Chondrogenic differentiation of NDF was studied in the presence or absence of hydrostatic compression under normal and hypoxic conditions.