Tubal Epithelial Cells (TuEC)

Tubal epithelial cells are isolated from the tubal tissue. The fallopian tubes are located on either side of the floor of the uterus and surround the upper edge of the broad ligament of the uterus. It extends from the two horns of the uterus to the left and right ovaries, and is the passageway for the egg cells to enter the uterus and the site where the egg is fertilized. Oviduct epithelial cells change with the menstrual cycle under the influence of ovarian estrogen and progesterone. Functional activity of estrogen to promote the growth of fallopian tube epithelial cells in late endometrial hyperplasia (pre-ovulation). The tubal epithelial cells were monolayer tall columnar cells. During endometrial hyperplasia (pre-ovulation), the epithelial cells are elevated and the cytoplasm of the secretory cells is filled with secretory particles. During the endometrial secretion phase, secretory cells release their secretions during apocrine secretion, thereby reducing epithelial cells. During menstruation and pregnancy, epithelial cells become shorter. The fallopian tube is an important site for the transport, storage and capacitation of eggs, as well as the adoption, transport, maturation, fertilization and early embryonic development of oocytes. In vitro culture of tubal epithelial cells can be used for feeding layer cells and co-culture with embryos to overcome the development retardation of embryos. Therefore, in vitro culture of tubal epithelial cells can not only further understand the factors affecting the change of reproductive microenvironment, but also establish a co-culture system of tubal epithelial cells and embryos to study the influence of tubal epithelial cells on the development of embryos in vitro.