≡

Kelch Like ECH Associated Protein 1 (KEAP1)

[Edit]

INrf2; KLHL19; Cytosolic inhibitor of Nrf2; Kelch-like protein 19

Kelch Like ECH Associated Protein 1 (KEAP1)
CCC L648
HGNC 23177
MGI 1858732
PubMed 24011591
RGD 621619
UniProt Q14145
Wiki KEAP1
KEAP1 encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.Kelch motifs of Keap1 interact with the Neh2 domain of Nrf2 and that this interaction is required for the repression of Nrf2 by Keap1. Fluorescence microscopy demonstrated that Keap1 is expressed in the cytoplasm with Nrf2.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPL648Hu01 Recombinant Kelch Like ECH Associated Protein 1 (KEAP1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAL648Hu01 Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) WB; IHC; ICC/IF
MAL648Hu21 Monoclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) WB; IHC; ICC; IP.
Assay Kits SEL648Hu ELISA Kit for Kelch Like ECH Associated Protein 1 (KEAP1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPL648Mu01 Recombinant Kelch Like ECH Associated Protein 1 (KEAP1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAL648Mu01 Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) WB; IHC; ICC; IP.
Assay Kits SEL648Mu ELISA Kit for Kelch Like ECH Associated Protein 1 (KEAP1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPL648Ra01 Recombinant Kelch Like ECH Associated Protein 1 (KEAP1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAL648Ra01 Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Kelch Like ECH Associated Protein 1 (KEAP1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit Customized Service Offer
  1. "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1."DNA Res. 2:167-174(1995) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Distinct cysteine residues in Keap1 are required for Keap1-dependent ubiquitination of Nrf2 and for stabilization of Nrf2 by chemopreventive agents and oxidative stress."Mol. Cell. Biol. 23:8137-8151(2003) [PubMed] [Europe PMC] [Abstract]
  6. "Fetal Alz-50 clone 1 interacts with the human orthologue of the Kelch-like Ech-associated protein."Biochemistry 43:12113-12122(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex."Mol. Cell. Biol. 24:10941-10953(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Ubiquitination of Keap1, a BTB-Kelch substrate adaptor protein for Cul3, targets Keap1 for degradation by a proteasome-independent pathway."J. Biol. Chem. 280:30091-30099(2005) [PubMed] [Europe PMC] [Abstract]
  9. "Nuclear oncoprotein prothymosin alpha is a partner of Keap1: implications for expression of oxidative stress-protecting genes."Mol. Cell. Biol. 25:1089-1099(2005) [PubMed] [Europe PMC] [Abstract]
  10. "Nrf1 is targeted to the endoplasmic reticulum membrane by an N-terminal transmembrane domain. Inhibition of nuclear translocation and transacting function."J. Biol. Chem. 281:19676-19687(2006) [PubMed] [Europe PMC] [Abstract]
  11. "PGAM5, a Bcl-XL-interacting protein, is a novel substrate for the redox-regulated Keap1-dependent ubiquitin ligase complex."J. Biol. Chem. 281:37893-37903(2006) [PubMed] [Europe PMC] [Abstract]
  12. "PGAM5 tethers a ternary complex containing Keap1 and Nrf2 to mitochondria."Exp. Cell Res. 314:1789-1803(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Ectodermal-neural cortex 1 down-regulates Nrf2 at the translational level."PLoS ONE 4:E5492-E5492(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  15. "Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites."Nature 502:254-257(2013) [PubMed] [Europe PMC] [Abstract]
  16. "Crystallization and initial crystallographic analysis of the Kelch domain from human Keap1."Acta Crystallogr. D 60:2346-2348(2004) [PubMed] [Europe PMC] [Abstract]
  17. "Conserved solvent and side-chain interactions in the 1.35 Angstrom structure of the Kelch domain of Keap1."Acta Crystallogr. D 61:1335-1342(2005) [PubMed] [Europe PMC] [Abstract]
  18. "Structure of the Keap1:Nrf2 interface provides mechanistic insight into Nrf2 signaling."EMBO J. 25:3605-3617(2006) [PubMed] [Europe PMC] [Abstract]
  19. "Structural basis for defects of Keap1 activity provoked by its point mutations in lung cancer."Mol. Cell 21:689-700(2006) [PubMed] [Europe PMC] [Abstract]
  20. "Dysfunctional KEAP1-NRF2 interaction in non-small-cell lung cancer."PLoS Med. 3:1865-1876(2006) [PubMed] [Europe PMC] [Abstract]
  21. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]