Uricase (UAO)

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UOX; UOXP; URICASE; UO; Urate Oxidase; Factor-Independent Urate Hydroxylase

Uricase (UAO)
A simple purification method of the Bacillus uricase (Uao) was newly developed. The gene coding for Uao with a C-terminal 6-histidine tag (Uao-HT) was constructed and overexpressed. Using the non-specific proteases, such as proteinase K, the tag was easily removed because Uao-HT includes its C-terminal region to be specifically cleaved by them.
Such treatment of Uao-HT with the proteases did not affect its enzymatic properties and enabled us to purify it from the crude extract with a single-step protocol; the cell lysate containing Uao-HT was mixed with the Ni ion-chelating magnetic beads and then the adsorbed enzyme was eluted with the proteinase K-containing buffer after untagged proteins were washed out. The isolated enzyme yielded a single band on SDS-PAGE and was fully active. This method is extremely useful for high-throughput purification of mutants because of compatibility with automation.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Complete Antigen of Uricase (UAO) Antigenic Transformation Customized Service Offer
Antibodies n/a Monoclonal Antibody to Uricase (UAO) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Uricase (UAO) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Uricase (UAO) CLIA Kit Customized Service Offer
n/a ELISA Kit for Uricase (UAO) ELISA Kit Customized Service Offer