ELISA Kit for Activin A (ACVA)
ACV-A; Activin Beta A Beta A Homodimer
- Product No.CEA001Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.94pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 630
For more details, please contact local distributors! US$ 2835
For more details, please contact local distributors! US$ 5355
For more details, please contact local distributors! US$ 44100
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Activin A (ACVA).
No significant cross-reactivity or interference between Activin A (ACVA) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Activin A (ACVA) and the recovery rates were calculated by comparing the measured value to the expected amount of Activin A (ACVA) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A (ACVA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A (ACVA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Activin A (ACVA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|13||Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte Dysfunction Ahajournals: Source|
|J. Pharmacogn||Article: Hksobf_2015_V46N2_167|
|United States Patent Application||Methods for Diagnosis and Treatment of Concussion or Brain Injury 2016:0103140|
|BMC Cancer.||Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. pubmed:27835986|
|Basic Clin Pharmacol Toxicol.||Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. pubmed:28032440|
|Basic & Clinical Pharmacology & Toxicology||Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling doi:10.1111|
|Journal of Immunology||MicroRNA-27b Modulates Inflammatory Response and Apoptosis during Mycobacterium tuberculosis Infection Pubmed:29661829|
|Journal of Vascular Surgery||Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissection Pubmed:29685513|
|Acta Biochimica et Biophysica Sinica||Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis 10.1093:abbs|
|Poultry Science||The effect of raw and fermented rapeseed cake on the metabolic parameters, immune status, and intestinal morphology of turkeys Pubmed:29917099|
|Food Bioscience||Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity 10.1016:j.fbio.2018.06.002|
|Current Analytical Chemistry||Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A 10.2174:2211556006666170525161225|
|Journal of Veterinary Internal Medicine||FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB :|
|Universal Journal of Pharmaceutical Research||SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY-YEMEN 10.22270:ujpr.v3i2.138|
|Involvement of RBP4 in diabetic atherosclerosis and the role of vitamin D intervention :|
|Clin Oral Investig.||Gingival crevicular fluid levels of human beta-defensin-1 in type 2 diabetes mellitus and periodontitis Pubmed:29713888|
|Myocardial fibrosis in hypertrophic cardiomyopathy :|
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|Duchenne Muscular Dystrophy||Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular Dystrophy Pubmed:29067667|
|Journal of Geriatric Oncology||Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors Pubmed: 30952517|
|clinica chimica acta||The performances of serum activins and follistatin in the diagnosis of ectopic pregnancy: A prospective case-control study Pubmed: 31647905|
|hindawi||The Association between the Activin A Serum Level and Carotid Intima-Media Thickness in Chronic Kidney Disease Patients|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA001Hu01||Recombinant Activin A (ACVA)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA001Hu01||Active Activin A (ACVA)||Cell culture; Activity Assays.|
|PAA001Hu01||Polyclonal Antibody to Activin A (ACVA)||WB; ICC; IHC-P; IHC-F; ELISA.|
|SEA001Hu||ELISA Kit for Activin A (ACVA)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEA001Hu||ELISA Kit for Activin A (ACVA)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA001Hu||CLIA Kit for Activin A (ACVA)||Chemiluminescent immunoassay for Antigen Detection.|