ELISA Kit for Angiotensin II (AngII)
- Product No.CEA005Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range24.69-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 8.89pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII).
No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Angiotensin II (AngII) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiotensin II (AngII) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II (AngII) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II (AngII) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin II (AngII) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Molecular medicine||Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition MolMed: 10_11_liu|
|The European Journal of Clinical Investigation||Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes Wiley: source|
|Immunopharmacology and Inflammation||Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid ScienceDirect: S0188440910001049|
|Nephrology||Effect of aggregated immunoglobulin A1 from immunoglobulin A nephropathy patients on nephrin expression in podocytes Wiley: source|
|PLoS One||Angiotensin II and Angiotensin-(1-7) in Paraventricular Nucleus Modulate Cardiac Sympathetic Afferent Reflex in Renovascular Hypertensive Rats PubMed: PMC3527547|
|BioMed Research International||Heterogeneous Downregulation of Angiotensin II AT1-A and AT1-B Receptors in Arterioles in STZ-Induced Diabetic Rat Kidneys Hindawi: 947506|
|European Heart Journal||Biglcan is beneficial in angiotensin II induced heart failure by preventing cardiac inflammation and remodeling improving LV function and mortality by preventing transdifferentiation of myofibroblasts Oxfordjournals: Source|
|Pflügers Archiv-European Journal of Physiology||SOD1 gene transfer into paraventricular nucleus attenuates hypertension and sympathetic activity in spontaneously hypertensive rats Pubmed: 23114721|
|Eur J Pharmacol.||Angiotensin II and vasopressin are involved in the defense system against anaphylactic hypotension in anesthetized rats Pubmed:24650734|
|J Mol Cell Cardiol.||Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunction Pubmed:25871829|
|Mol Med Rep.||High glucose induces activation of the local renin?angiotensin system in glomerular endothelial cells Pubmed:24337709|
|Mol Med Rep.||Angiotensin II promotes the progression of human gastric cancer Pubmed:24424956|
|PLOS ONE||Intermedin in the Paraventricular Nucleus Attenuates Cardiac Sympathetic Afferent Reflex in Chronic Heart Failure Rats Pubmed:24709972|
|Journal of Vascular Surgery||Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice Pubmed:25037606|
|Biochim Biophys Acta.||Cathepsin G activity lowers plasma LDL and reduces atherosclerosis Pubmed:25092171|
|Acta Physiologica||Superoxide anions in the paraventricular nucleus mediate cardiac sympathetic afferent reflex in insulin resistance rats Pubmed:25307720|
|Neuroscience Letters||Administration of exogenous 1,25(OH)2D3 normalizes overactivation of the central renin-angiotensin system in 1α(OH)ase knockout mice Pubmed:25576706|
|european heart journal||A background Ca2+ PubMed: 26069213|
|Life Sci||Cardioprotective effects of baicalein on heart failure via modulation of Ca2 PubMed: 26706290|
|Physiological Research||Renal Sympathetic Denervation Improves Cardiac Dysfunction PubMed: 25804096|
|Nutrition||Effects of Rubus occidentalis extract on blood pressure in subjects with prehypertension: randomized, double-blinded, placebo controlled clinical trial PubMed: 26740254|
|J Am Soc Hypertens||Angiotensin-(1–7) enhances the effects of angiotensin II on the cardiac sympathetic afferent reflex and sympathetic activity in rostral ventrolateral medulla in renovascular hypertensive rats PubMed: 26428223|
|PLoS One.||Up ReCavia (Guinea pig )lation of cystathione γ lyase and Hydrogen Sulphide in the Myocardium Inhibits the Progression of Isoproterenol–Caffeine Induced Left Ventricular Hypertrophy in Wistar Kyoto Rats Pubmed:26963622|
|Nutrition||Effects of Rubus occidentalis extract on blood pressure in patients with prehypertension: Randomized, double-blinded, placebo-controlled clinical trial Pubmed:26740254|
|Journal of Atherosclerosis and Thrombosis||Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase ReCavia (Guinea pig )lation in Human Carotid Atheroma in vitro Pubmed:26947595|
|Mol Neurobiol||A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats Pubmed:26867655|
|Life Sci||Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro Pubmed:26706290|
|PLoS One.||Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training. pubmed:27880816|
|Oxidative Medicine and Cellular Longevity||Epigallocatechin-3-gallate Attenuates Renal Damage by Suppressing Oxidative Stress in Diabetic db/db Mice pubmed:27698952|
|Journal of the Renin-Angiotensin-Aldosterone System||Angiotensin type 2 receptor null mice express reduced levels of renal angiotensin converting enzyme-2/angiotensin (1-7)/Mas receptor and exhibit greater high-fat diet-induced kidney injury 1470320316661871.abstract|
|Microvascular Research||Pleiotropic effects of survivin in vascular endothelial cells. pubmed:27373518|
|PLOS ONE||Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. pubmed:27391973|
|journal of pharmacological sciences||β-Aminoisobutyric acid ameliorates the renal fibrosis in mouse obstructed kidneys via inhibitionof renal fibroblast activation and fibrosis. pubmed:28433566|
|Experimental Animals||Ginsenoside Rb1 improves cardiac function and remodeling in heart failure. pubmed:28367863|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|CEA005Ra||ELISA Kit for Angiotensin II (AngII)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA005Ra11||ELISA Kit DIY Materials for Angiotensin II (AngII)||Main materials for "Do It (ELISA Kit) Yourself".|