ELISA Kit for Dihydrotestosterone (DHT)
5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone
- Product No.CEA443Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range30.9-2500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 13.3pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Dihydrotestosterone (DHT).
No significant cross-reactivity or interference between Dihydrotestosterone (DHT) and analogues was observed.
Matrices listed below were spiked with certain level of Dihydrotestosterone (DHT) and the recovery rates were calculated by comparing the measured value to the expected amount of Dihydrotestosterone (DHT) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dihydrotestosterone (DHT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dihydrotestosterone (DHT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Dihydrotestosterone (DHT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Internation Scholarly Research Network||Herbicide Metolachlor Causes Changes in Reproductive Endocrinology of Male Wistar Rats Isrn: 130846|
|Journal of American Science||Metabolic Changes and Hormonal Disturbances in Polycystic Ovarian Syndrome Rats and the Amelioration Effects of Metformin and/or Cinnamon Extraction Jofamericanscience: Source|
|Journal of Ethnopharmacology||Treatment of benign prostatic hyperplasia with Croton membranaceus in an experimental animal model Pubmed:25256687|
|Toxicol Ind Health||Oral administration of low-dose bisphenol A promotes proliferation of ventral prostate and upregulates prostaglandin D2 PubMed: 26088557|
|PPAR Research||Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362|
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