ELISA Kit for Testosterone (Testo)

T; Testesteron; Testeronun

Specificity

This assay has high sensitivity and excellent specificity for detection of Testosterone (Testo).
No significant cross-reactivity or interference between Testosterone (Testo) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Testosterone (Testo) and the recovery rates were calculated by comparing the measured value to the expected amount of Testosterone (Testo) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)98-105101
EDTA plasma(n=5)98-105101
heparin plasma(n=5)98-105101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Testosterone (Testo) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Testosterone (Testo) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-104%88-96%91-99%80-98%
EDTA plasma(n=5)87-98%99-105%92-101%90-99%
heparin plasma(n=5)80-91%91-99%81-91%90-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1Assay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
Reagent Diluent1×300µLStop Solution1×6mL
TMB Substrate1×9mLInstruction manual1
Wash Buffer (30 × concentrate)1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Testosterone (Testo) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Testosterone (Testo) and unlabeled Testosterone (Testo) (Standards or samples) with the pre-coated antibody specific to Testosterone (Testo). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Testosterone (Testo) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Testosterone (Testo) in the sample.

GIVEAWAYS

INCREMENT SERVICES

MagazineReference
The Tohoku Journal of Experimental MedicineAndrogen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature RatsJstage: source
Plos oneInsulin Receptor Substrate 2 Is Required for Testicular DevelopmentPubMed: PMC3669358
International Journal of Molecular and Cellular MedicineCentral effects of camphor on GnRH and sexual hormones in male ratPubmed: 24551777
Urologia internationalisEffects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in RatsPubmed: 24051982
International Braz J UrolThe effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats.Pubmed: 24642156
Journal of Materials Chemistry BNanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineeringRsc: Source
Biochemical and Biophysical Research CommunicationsCritical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesisScienceDirect: S0006291X14001843
PLoS One.Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostatePubmed:Pmc4100933
Reproductive ToxicologyCarbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspringPubmed:24126190
Physiology &amp; BehaviorThe impact of maternal consumption of cafeteria diet on reproductive function in the offspringPubmed:24631302
Int Braz J Urol. The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in ratsPubmed:24642156
Environmental ResearchPerfluorooctane sulfonate effects on the reproductive axis in adult male ratsPubmed:25171141
Acta Cir Bras.Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model.Pubmed:25185055
Genet Mol Biol. Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertilityPubmed:25505846
Turkish Journal of UrologyApoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapyTurkishjournalofurology:Source
Int J Clin Exp MedEffects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetesPubMed: 26379831
Int Braz J UrolMetabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult RatsPubMed: 26200553
Advances in Environmental BiologyPlasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic ExtractAensiweb: Aeb
international journal of environmental research and public health Elevated Serum Bisphenol A Level in Patients with Dilated CardiomyopathyPubMed: 25996886
Environ ToxicolEffect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis periodPubMed: 25782374
J EndocrinolTestosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarctionPubMed: 25770118
Acta Pharmaceutica Sinica BPreparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrhscience:S2211383516301812
Plos OneAssociation between Polycystic Ovary Syndrome and Cavia (Guinea pig )t MicrobiotaPubmed:27093642
TheriogenologyExpression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle countPubmed:26948295
PPAR ResearchTestosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARαPubmed:27413362
TURKISH JOURNAL OF MEDICAL SCIENCESTHE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATSfileID:693211
Int J Mol Sci.Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in MicePubmed:26771609
Catalog No.Related products for research use of Pan-species (General) Organism speciesApplications
CPA458Ge11BSA Conjugated Testosterone (Testo)Immunogen; SDS-PAGE; WB.
CPA458Ge21OVA Conjugated Testosterone (Testo)Immunogen; SDS-PAGE; WB.
PAA458Ge01Polyclonal Antibody to Testosterone (Testo)WB; IHC; ICC; IP.
LAA458Ge71Biotin-Linked Polyclonal Antibody to Testosterone (Testo)WB; IHC; ICC.
MAA458Ge21Monoclonal Antibody to Testosterone (Testo)WB; IHC; ICC; IP.
IEA458GeInstant ELISA Kit for Testosterone (Testo)Enzyme-linked immunosorbent assay for Antigen Detection.
CEA458GeELISA Kit for Testosterone (Testo)Enzyme-linked immunosorbent assay for Antigen Detection.
CCA458GeCLIA Kit for Testosterone (Testo)Chemiluminescent immunoassay for Antigen Detection.
KSA458Ge11ELISA Kit DIY Materials for Testosterone (Testo)Main materials for "Do It (ELISA Kit) Yourself".