ELISA Kit for Testosterone (Testo)
T; Testesteron; Testeronun
- Product No.CEA458Ge
- Organism SpeciesPan-species (General)Same name, Different species.
- Sample Typeserum, plasma, urine and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range123.5-10000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 53.7pg/mL.
- DownloadInstruction Manual
- FOB US$ 279
For negotiated price and more details, please contact local distributors! US$ 398
For negotiated price and more details, please contact local distributors! US$ 1791
For negotiated price and more details, please contact local distributors! US$ 3383
For negotiated price and more details, please contact local distributors! US$ 27860
For negotiated price and more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Testosterone (Testo).
No significant cross-reactivity or interference between Testosterone (Testo) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Testosterone (Testo) and the recovery rates were calculated by comparing the measured value to the expected amount of Testosterone (Testo) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Testosterone (Testo) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Testosterone (Testo) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Testosterone (Testo) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Testosterone (Testo) and unlabeled Testosterone (Testo) (Standards or samples) with the pre-coated antibody specific to Testosterone (Testo). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Testosterone (Testo) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Testosterone (Testo) in the sample.
|The Tohoku Journal of Experimental Medicine||Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature RatsJstage: source|
|Plos one||Insulin Receptor Substrate 2 Is Required for Testicular DevelopmentPubMed: PMC3669358|
|International Journal of Molecular and Cellular Medicine||Central effects of camphor on GnRH and sexual hormones in male ratPubmed: 24551777|
|Urologia internationalis||Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in RatsPubmed: 24051982|
|International Braz J Urol||The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats.Pubmed: 24642156|
|Journal of Materials Chemistry B||Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineeringRsc: Source|
|Biochemical and Biophysical Research Communications||Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesisScienceDirect: S0006291X14001843|
|PLoS One.||Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostatePubmed:Pmc4100933|
|Reproductive Toxicology||Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspringPubmed:24126190|
|Physiology & Behavior||The impact of maternal consumption of cafeteria diet on reproductive function in the offspringPubmed:24631302|
|Int Braz J Urol.||The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in ratsPubmed:24642156|
|Environmental Research||Perfluorooctane sulfonate effects on the reproductive axis in adult male ratsPubmed:25171141|
|Acta Cir Bras.||Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model.Pubmed:25185055|
|Genet Mol Biol.||Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertilityPubmed:25505846|
|Turkish Journal of Urology||Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapyTurkishjournalofurology:Source|
|Int J Clin Exp Med||Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetesPubMed: 26379831|
|Int Braz J Urol||Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult RatsPubMed: 26200553|
|Advances in Environmental Biology||Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic ExtractAensiweb: Aeb|
|international journal of environmental research and public health||Elevated Serum Bisphenol A Level in Patients with Dilated CardiomyopathyPubMed: 25996886|
|Environ Toxicol||Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis periodPubMed: 25782374|
|J Endocrinol||Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarctionPubMed: 25770118|
|Acta Pharmaceutica Sinica B||Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrhscience:S2211383516301812|
|Plos One||Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t MicrobiotaPubmed:27093642|
|Theriogenology||Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle countPubmed:26948295|
|PPAR Research||Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARαPubmed:27413362|
|TURKISH JOURNAL OF MEDICAL SCIENCES||THE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATSfileID:693211|
|Int J Mol Sci.||Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in MicePubmed:26771609|
|Catalog No.||Related products for research use of Pan-species (General) Organism species||Applications|
|CPA458Ge11||BSA Conjugated Testosterone (Testo)||Immunogen; SDS-PAGE; WB.|
|CPA458Ge21||OVA Conjugated Testosterone (Testo)||Immunogen; SDS-PAGE; WB.|
|PAA458Ge01||Polyclonal Antibody to Testosterone (Testo)||WB; IHC; ICC; IP.|
|LAA458Ge71||Biotin-Linked Polyclonal Antibody to Testosterone (Testo)||WB; IHC; ICC.|
|MAA458Ge21||Monoclonal Antibody to Testosterone (Testo)||WB; IHC; ICC; IP.|
|IEA458Ge||Instant ELISA Kit for Testosterone (Testo)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEA458Ge||ELISA Kit for Testosterone (Testo)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CCA458Ge||CLIA Kit for Testosterone (Testo)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA458Ge11||ELISA Kit DIY Materials for Testosterone (Testo)||Main materials for "Do It (ELISA Kit) Yourself".|