ELISA Kit for Cortisol (Cor)
- Product No.CEA462Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample TypeSerum, plasma, urine and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.84ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 612
For more details, please contact local distributors! US$ 2754
For more details, please contact local distributors! US$ 5202
For more details, please contact local distributors! US$ 42840
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Cortisol (Cor).
No significant cross-reactivity or interference between Cortisol (Cor) and analogues was observed.
Matrices listed below were spiked with certain level of Cortisol (Cor) and the recovery rates were calculated by comparing the measured value to the expected amount of Cortisol (Cor) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cortisol (Cor) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cortisol (Cor) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cortisol (Cor) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Research in Veterinary Science||Use of infrared thermography to assess the influence of high environmental temperature on rabbits ScienceDirect: S0034528813001380|
|British Journal of Nutrition||Identification of sociodemographic and clinical factors associated with the levels of human b-defensin-1 and human b-defensin-2 in the human milk of Han Chinese Pubmed:24124699|
|Theriogenology||Daily exposure to summer circadian cycles affects spermatogenesis, but not fertility in an in vivo rabbit model Pubmed:25442388|
|Animal Production Science||Effect of high temperatures on breeding rabbit behaviour Publish:Source|
|JBCD||Assessment of cortisol as salivary psychological stress marker in relation to temporomandibular disorders among a sample of dental students Index.Php: Jbcd|
|International Journal of Immunopathology & Pharmacology||Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats PubMed: 26684638|
|IET Micro & Nano Letters||Surface plasmon resonance immunoassay for cortisol determination with a self-assembling denaturalised bovine serum albumin layer on surface plasmon resonance chip xpls:7393900|
|Optoelectronics Letters||Fabrication and characterization of SPR chips with the modified bovine serum albumin article:10.1007|
|Journal of Dairy Science||Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves publication:306322503|
|Acta Veterinaria Hungarica||Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation acve-2017-0006|
|BMC Veterinary Research||Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … Pubmed:29540178|
|Indian Journal of Animal Sciences||Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition :|
|Biological Rhythm Research||Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation …|
|International Journal of Current Microbiology and Applied Sciences||Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons|
|Journal of Animal & Plant Sciences||A study on certain blood biomarkers of pregnant Cholistani cows.|
|Animals||The Use of Infrared Thermography (IRT) as Stress Indicator in Horses Trained for Endurance: A Pilot Study Pubmed: 30866503|
|Catalog No.||Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species||Applications (RESEARCH USE ONLY!)|
|CEA462Rb||ELISA Kit for Cortisol (Cor)||Enzyme-linked immunosorbent assay for Antigen Detection.|