ELISA Kit for Thyroid Stimulating Hormone (TSH)

Thyrotropin

Specificity

This assay has high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone (TSH).
No significant cross-reactivity or interference between Thyroid Stimulating Hormone (TSH) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Thyroid Stimulating Hormone (TSH) and the recovery rates were calculated by comparing the measured value to the expected amount of Thyroid Stimulating Hormone (TSH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-102 99
EDTA plasma(n=5) 93-101 97
heparin plasma(n=5) 87-99 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thyroid Stimulating Hormone (TSH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-103% 79-97% 79-102% 78-99%
EDTA plasma(n=5) 82-101% 96-105% 98-105% 85-93%
heparin plasma(n=5) 94-102% 79-96% 94-102% 86-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Blood Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID Pubmed: 22184407
Life Science Journal Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies Lifesciencesite: Source
Thyroid Impact of Metformin and Compound C on NIS expression and iodine uptake in vitro and in vivo: a role for CRE in AMPK modulation of thyroid function Pubmed: 23819433
Journal of Functional Foods Effect of lettuce biofortified with iodine by soil fertilization on iodine concentration in various tissues and selected biochemical parameters in serum of Wistar rats Sciencedirect:S1756464615000894
J Endocrinol. Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis Pubmed:24928937
journal of lipid research Thyroid-stimulating hormone decreases HMG-CoA reductase phosphorylation via AMP-activated protein kinase in the liver PubMed: 25713102
Int J Clin Exp Med Lead exposure causes thyroid abnormalities in diabetic rats PubMed: 26221254
Jubilant Drax Image Perinatal Iron Deficiency-Induced Hypothyroxinemia Impairs Early Brain Development Regardless of Normal Iron Levels in the Neonatal Brain Pubmed:27231981
PLoS One. The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats Pubmed:27043135
journal of bone and mineral research Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis. pubmed:27589370
Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384
Sci Rep Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267
Bulletin of the World Health Organization Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders handle:123456789
International Journal of Peptide Research and Therapeutics Effect of Intracerebroventricular Administration of Apelin-13 on the Hypothalamus–Pituitary–Thyroid Axis and Peripheral Uncoupling Proteins article:10.1007
Tropical Journal of Pharmaceutical Research Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats 10.4314/tjpr.v16i5.9
30 The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. pubmed:28546511
NITRIC OXIDE-BIOLOGY AND CHEMISTRY Voluntary exercise training restores anticontractile effect of NO in coronary arteries of adult rats with antenatal/early postnatal hypothyroidism Pubmed:29307633
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355
Neuroscience Letter Central irisin administration suppresses thyroid hormone production but increases energy consumption in rats Pubmed:29574218
Journal of Neuroinflammation Hashimoto's thyroiditis induces neuroinflammation and emotional alterations in euthyroid mice Pubmed: 30373627
Cell Reports Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Pubmed: 30404008
Journal of Diabetes Research The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice
Endocrine Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity Pubmed: 31056723
Oxidative Medicine and Cellular Longevity Oxidative Stress Produced by Hyperthyroidism Status Induces the Antioxidant Enzyme Transcription through the Activation of the Nrf-2 Factor in Lymphoid Tissues of …
Reproductive Biology and Endocrinology Hashimoto's thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid mice Pubmed: 31729993
Gli Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice Pubmed: 32173924
Clinical Immunology The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice Pubmed: 31982645
Nature Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Pubmed: 33177714
Thyroid Generation and Characterization of a New Resistance to Thyroid Hormone Mouse Model with Thyroid Hormone Receptor Alpha Gene Mutation Pubmed: 32924834
Thyroid Hashimoto's thyroiditis induces hippocampus-dependent cognitive alterations by impairing astrocytes in euthyroid mice Pubmed: 32907517
Int Immunopharmacol Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy Pubmed: 32442899
Brazilian Journal of Development Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposição materna ao triclosan causa …
Comparaison des marqueurs hormonaux et histomorphométriques chez la souris un modèle d'hypothyroïdie après 28 jours de traitement au PTU par voie alimentaire.
Andrologia Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine©\treated rat dams during pregnancy and lactation 33433934
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart 33375430
Biomedicines Role of Kallikrein 7 in Body Weight and Fat Mass Regulation. Biomedicines 2021, 9, 131 33572949
Mol Neurobiol Maternal Subclinical Hypothyroidism in Rats Impairs Spatial Learning and Memory in Offspring by Disrupting Balance of the TrkA/p75 NTR Signal Pathway 33966253
Clin Epigenetics Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism 34092241
Pharmaceutics Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays
BRITISH JOURNAL OF NUTRITION Effects of iodine excess on serum thyrotropin-releasing hormone levels and type 2 deiodinase in the hypothalamus of Wistar rats 34250878
Gene Therapy Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR2A vector 34599290
NatCommun Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis 34876572
Int J Mol Sci The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism Pubmed:35163456
Molecular Biology Reports Effects of meteorin-like hormone on endocrine function of hypothalamo-hypophysial system and peripheral uncoupling proteins in rats Pubmed:35332411
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
SPA463Hu02 Synthetic Thyroid Stimulating Hormone (TSH) Immunogen; Blocking Peptide.
CPA463Hu21 OVA Conjugated Thyroid Stimulating Hormone (TSH) Immunogen; SDS-PAGE; WB.
CPA463Hu22 OVA Conjugated Thyroid Stimulating Hormone (TSH) Immunogen; SDS-PAGE; WB.
PAA463Hu01 Polyclonal Antibody to Thyroid Stimulating Hormone (TSH) WB; IHC; ICC; IP.
PAA463Hu08 Polyclonal Antibody to Thyroid Stimulating Hormone (TSH) WB; IHC; ICC; IP.
PAA463Hu09 Polyclonal Antibody to Thyroid Stimulating Hormone (TSH) WB; IHC; ICC; IP.
IEA463Hu Instant ELISA Kit for Thyroid Stimulating Hormone (TSH) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA463Hu ELISA Kit for Thyroid Stimulating Hormone (TSH) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA463Hu ELISA Kit for Thyroid Stimulating Hormone (TSH) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA463Hu Multiplex Assay Kit for Thyroid Stimulating Hormone (TSH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA463Hu11 ELISA Kit DIY Materials for Thyroid Stimulating Hormone (TSH) Main materials for "Do It (ELISA Kit) Yourself".