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ELISA Kit for Immunoglobulin G (IgG)


This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG).
No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Immunoglobulin G (IgG) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G (IgG) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-94 85
EDTA plasma(n=5) 84-92 88
heparin plasma(n=5) 80-95 86


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G (IgG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-99% 97-105% 80-91% 78-97%
EDTA plasma(n=5) 79-97% 90-104% 87-96% 81-99%
heparin plasma(n=5) 85-102% 82-96% 78-92% 85-98%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
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Journal of Animal and Veterinary Advances Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus Medwell: 2010594600
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source
Poultry Science Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 Pubmed: 24570422
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BioMed Research International The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Hindawi:Source
Journal of Proteomics Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection Pubmed:24576640
Int J Occup Environ Health. Liver functions in silica-exposed workers in Egypt: possible role of matrix remodeling and immunological factors Pubmed:24999850
Food &amp; Function Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice Pubmed:25308407
Oncology Reports Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer Pubmed:25529861
Biomed Res Int The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling PubMed: 26161394
Protein Pept Lett Stabilization of Human Immunoglobulin G Encapsulated within Biodegradable Poly (Cyclohexane-1, 4-diyl Acetone Dimethylene Ketal) (PCADK)/ Poly (Lactic-co-Glycolic Acid) (PLGA) Blend Microspheres PubMed: 26268563
Electrophoresis Growth promotion in pigs by oxytetracycline coincides with down reCavia (Guinea pig )lation of serum inflammatory parameters and of hibernation‐associated protein HP‐27 Pubmed:26914286
Neuropeptides Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro. Pubmed:26707636
Archives of Biological Sciences Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine pid:46641600021Z
journal of biotechnology Expression of α-1,6-fucosyltransferase (FUT8) in rice grain and immunogenicity evaluation of plant-specific glycans. pubmed:28013072
Sci Rep.  Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. pubmed:28220870
Toxicology Research Effect of dietary protein or calcium supplement on the expression of collagen I and dentine phosphoprotein of rats with dental fluorosis C6TX00288A
ntervirology Exposure of Piglets to Enteroviruses Investigated by an Immunoassay Based on the EV-G1 VP4 Peptide pubmed:27694750
Biomedicine & Pharmacotherapy Icariin promotes osteogenic differentiation of rat bone marrow stromal cells by activating the ERα-Wnt/β-catenin signaling pathway. pubmed:27764755
Food and Agricultural Immunology Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers 10.108:09540105.2017.1311308
Biomedicine & Pharmacotherapy In vitro and in vivo immunomodulatory potential of Pedicularis longiflora and Allium carolinianum in alloxan-induced diabetes in rats pubmed:29091887
Journal of Biochemical and Molecular Toxicology Fluoride or/and aluminum induced toxicity in guinea pig teeth with the low expression of dentine phosphoprotein 10.1002/jbt.21912
International Journal of Science and Research Impact of Silver Nanoparticles on Immune Responses as Vaccine against Activated Staphylococcus aureus Bacteria ISSN: 2319-7064
Molecular Medicine Reports Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. pubmed:28586019
Revista Brasileira de Zootecnia Influence of eggshell colour on egg yolk antibody level, incubation results, and growth in broiler breeders 10.1590/s1806-92902017000200004 
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