ELISA Kit for Immunoglobulin G (IgG)
- Product No.CEA544Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range1.23-100ug/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.59ug/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 418
For more details, please contact local distributors! US$ 1881
For more details, please contact local distributors! US$ 3553
For more details, please contact local distributors! US$ 29260
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG).
No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Immunoglobulin G (IgG) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G (IgG) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G (IgG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 5 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450 nm immediately.
|Journal of Animal and Veterinary Advances||Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine Medwell: 2010610616|
|Journal of Animal and Veterinary Advances||Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus Medwell: 2010594600|
|international Journal of food and nutritional sciences||EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source|
|Poultry Science||Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 Pubmed: 24570422|
|Pharmaceutical Development and Technology||Foot and Mouth Disease virus-loaded fungal chitosan nanoparticles for intranasal administration: impact of formulation on physicochemical and immunological characteristics Pubmed: 23590209|
|BioMed Research International||The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Hindawi:Source|
|Journal of Proteomics||Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection Pubmed:24576640|
|Int J Occup Environ Health.||Liver functions in silica-exposed workers in Egypt: possible role of matrix remodeling and immunological factors Pubmed:24999850|
|Food & Function||Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice Pubmed:25308407|
|Oncology Reports||Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer Pubmed:25529861|
|Biomed Res Int||The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling PubMed: 26161394|
|Protein Pept Lett||Stabilization of Human Immunoglobulin G Encapsulated within Biodegradable Poly (Cyclohexane-1, 4-diyl Acetone Dimethylene Ketal) (PCADK)/ Poly (Lactic-co-Glycolic Acid) (PLGA) Blend Microspheres PubMed: 26268563|
|Electrophoresis||Growth promotion in pigs by oxytetracycline coincides with down reCavia (Guinea pig )lation of serum inflammatory parameters and of hibernation‐associated protein HP‐27 Pubmed:26914286|
|Neuropeptides||Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro. Pubmed:26707636|
|Archives of Biological Sciences||Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine pid:46641600021Z|
|Asian J Pharm Clin Res||INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS journals:3790|
|journal of biotechnology||Expression of α-1,6-fucosyltransferase (FUT8) in rice grain and immunogenicity evaluation of plant-specific glycans. pubmed:28013072|
|Sci Rep.||Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. pubmed:28220870|
|Toxicology Research||Effect of dietary protein or calcium supplement on the expression of collagen I and dentine phosphoprotein of rats with dental fluorosis C6TX00288A|
|ntervirology||Exposure of Piglets to Enteroviruses Investigated by an Immunoassay Based on the EV-G1 VP4 Peptide pubmed:27694750|
|Biomedicine & Pharmacotherapy||Icariin promotes osteogenic differentiation of rat bone marrow stromal cells by activating the ERα-Wnt/β-catenin signaling pathway. pubmed:27764755|
|Food and Agricultural Immunology||Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers 10.108:09540105.2017.1311308|
|Biomedicine & Pharmacotherapy||In vitro and in vivo immunomodulatory potential of Pedicularis longiflora and Allium carolinianum in alloxan-induced diabetes in rats pubmed:29091887|
|Journal of Biochemical and Molecular Toxicology||Fluoride or/and aluminum induced toxicity in guinea pig teeth with the low expression of dentine phosphoprotein 10.1002/jbt.21912|
|International Journal of Science and Research||Impact of Silver Nanoparticles on Immune Responses as Vaccine against Activated Staphylococcus aureus Bacteria ISSN: 2319-7064|
|Molecular Medicine Reports||Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. pubmed:28586019|
|Revista Brasileira de Zootecnia||Influence of eggshell colour on egg yolk antibody level, incubation results, and growth in broiler breeders 10.1590/s1806-92902017000200004|
|Water Research||Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model Pubmed:29655095|
|Clinical Immunology||Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis Pubmed:30056130|
|Food and Agricultural Immunology||Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers 10.1080:09540105.2018.1457013|
|Journal of Molecular Neuroscience||A Novel scFv Anti-Aβ Antibody Reduces Pathological Impairments in APP/PS1 Transgenic Mice via Modulation of Inflammatory Cytokines and Aβ-related Enzymes Pubmed:30062438|
|Biological Trace Element Research||Supplementation of Cr Methionine During Dry Period of Dairy Cows and Its Effect on Some Production and Biochemical Parameters During Early Lactation and on … Pubmed:29497999|
|Probiotics Antimicrob Proteins||Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a … Pubmed:29305686|
|SAGE Open Medicine||Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes Pubmed:29623201|
|Journal of Biological Chemistry||Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids Pubmed: 30237167|
|ACS Applied Materials & Interfaces||Fabrication and characterization of core-shell nanofibers using a next-generation airbrush for biomedical applications Pubmed: 30433758|
|Molecular Human Reproduction||Phenotypic variations in transferred progeny due to genotype of surrogate mother: Surrogate mother modulates transferred progeny Pubmed: 30445548|
|Bioscience, Biotechnology, and Biochemistry||In vitro and in vivo cancer cell apoptosis triggered by competitive binding of Cinchona alkaloids to the RING domain of TRAF6|
|Journal of proteome research||Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection|
|International Immunopharmacology||Ginsenoside Rg3 improves cyclophosphamide-induced immunocompetence in Balb/c mice Pubmed: 30974284|
|Utjecaj dodatka selena u prihrani na hematološku sliku i biokemijske pokazatelje u jelena lopatara (Dama dama L.)|
|Biogerontology||Age-related chemokine alterations affect IgA secretion and gut immunity in female mice Pubmed: 32277312|
|International Journal of Biological Macromolecules||Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickens Pubmed: 32224178|
|Pharmaceutics||Efficacy of Local Minocycline Agents in Treating Peri-Implantitis: An Experimental In Vivo Study in Beagle Dogs Pubmed: 33114168|
|INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES||Physicochemical characterization and immunomodulatory activity of sulfated Chinese yam polysaccharide Pubmed: 33010421|
|Pharmaceutics||Locally Applied Slow-Release of Minocycline Microspheres in the Treatment of Peri-Implant Mucositis: An Experimental In Vivo Study Pubmed: 32708741|
|BIOLOGICAL TRACE ELEMENT RESEARCH||Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle Pubmed: 32583225|
|Molecular Medicine Reports||Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism Pubmed: 33000198|
|South African Journal of Botany||Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo|
|Journal of Functional Foods||Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts|
|Italian Journal of Animal Science||Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the …|
|Regenerative Biomaterials||A comparative study of autogenous, allograft and artificial bone substitutes on bone regeneration and immunotoxicity in rat femur defect model|
|ACS APPLIED BIO MATERIALS||Curcumin loaded nanostructure hybrid lipid capsules for co-eradication of breast cancer and cancer stem cells with enhanced anticancer efficacy|
|博士论文||Women Scientist Scientist Mentor|
|sensors and actuators b-chemical||A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate|