ELISA Kit for Orexin A (OXA)
- Product No.CEA607Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.99pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 630
For more details, please contact local distributors! US$ 2835
For more details, please contact local distributors! US$ 5355
For more details, please contact local distributors! US$ 44100
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Orexin A (OXA).
No significant cross-reactivity or interference between Orexin A (OXA) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Orexin A (OXA) and the recovery rates were calculated by comparing the measured value to the expected amount of Orexin A (OXA) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Orexin A (OXA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Orexin A (OXA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Orexin A (OXA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Frontiers in Neurology||Neuro-Glial and Systemic Mechanisms of Pathological Responses in Rat Models of Primary Blast Overpressure Compared to “Composite” Blast PubMed: PMC3275793|
|Neurological Sciences||Pentylenetetrazol-induced seizures are exacerbated by sleep deprivation through orexin receptor-mediated hippocampal cell proliferation Pubmed: 23852314|
|Peptides||Orexin-A-induced ERK1/2 activation reverses impaired spatial learning and memory in pentylenetetrazol-kindled rats via OX1R-mediated hippocampal neurogenesis. Pubmed: 24321199|
|Alcohol Alcohol||Orexin in Patients with Alcohol Dependence Treated for Relapse Prevention: A Pilot Study PubMed: 26597795|
|Indian Journal of Medical Research and Pharmaceutical Sciences||Evaluation of Antidiabetic And Insulintropic Potential of Nigella Sativa Seeds Water Extract and /or Alpha Lipoic Acid in Type 2 Diabetic Rats Issues%20Pdf: Vol.2|
|J Clin Res Pediatr Endocninol||Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children PubMed: 26831553|
|Neural Regen Res||Resuscitation therapy for traumatic brain injury-induced coma in rats: mechanisms of median nerve electrical stimulation PubMed: 26170820|
|Mol Med Rep||Wake-promoting actions of median nerve stimulation in TBI-induced coma: An investigation of orexin-A and orexin receptor 1 in the hypothalamic region PubMed: 26059340|
|Mol Neurobiol||Application of the Co-culture Membrane System Pointed to a Protective Role of Catestatin on Hippocampal Plus Hypothalamic Neurons Exposed to Oxygen and Glucose Deprivation article:10.1007|
|Neural Regeneration Research||Wake-promoting effects of vagus nerve stimulation after traumatic brain injury: upregulation of orexin-A and orexin receptor type 1 expression in the prefrontal … Pubmed:29557373|
|Journal of Translational Medicine||Recurrent circadian fasting (RCF) improves blood pressure, biomarkers of cardiometabolic risk and regulates inflammation in men Pubmed: 31426866|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|CEA607Hu||ELISA Kit for Orexin A (OXA)||Enzyme-linked immunosorbent assay for Antigen Detection.|