ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI)

NTX-I; NTX1

Specificity

This assay has high sensitivity and excellent specificity for detection of Cross Linked N-Telopeptide Of Type I Collagen (NTXI).
No significant cross-reactivity or interference between Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-101 89
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 78-98 81

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked N-Telopeptide Of Type I Collagen (NTXI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked N-Telopeptide Of Type I Collagen (NTXI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-99% 95-103% 88-97% 85-94%
EDTA plasma(n=5) 95-102% 84-93% 98-105% 87-104%
heparin plasma(n=5) 88-99% 90-98% 98-105% 95-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Nutrition Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacity ScienceDirect: S0899900709004717
Bone Differential mRNA expression profiles in proximal tibia of aged rats in response to ovariectomy and low-Ca diet ScienceDirect: S875632820800776X
Journal of Orthopaedic Research Promotion of bone formation by naringin in a titanium particle‐induced diabetic murine calvarial osteolysis model Wiley: Source
The Journal of Clinical Endocrinology & Metabolism A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular Risk PubMed: PMC3462931
Acta Paediatr.? Biochemical markers of bone resorption are present in human milk: implications for maternal and neonatal bone metabolism Pubmed:25109232
PLoS One. An NMR Metabolomic Study on the Effect of Alendronate in Ovariectomized Mice Pubmed:25184758
J Bone Miner Res Strontium Ranelate Reduces the Fracture Incidence in a Growing Mouse Model of Osteogenesis Imperfecta PubMed: 26679066
Journal of Functional Foods Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561
Archives of Oral Biology Impact of a chronic smoking habit on the osteo-immunoinflammatory mediators in the peri-implant fluid of clinically healthy dental implants. pubmed:27328151
Journal of Periodontology Impact of a triclosan‐containing toothpaste during the progression of experimental peri‐implant mucositis: Clinical parameters and local pattern of osteo … Pubmed:29520826
Clinical oral implants research Triclosan‐containing fluoride toothpaste on clinical parameters and osteo‐inflammatory mediators when applied in a stent during experimental peri‐implant mucositis … Pubmed: 30659666
Molecular Therapy-Nucleic Acids AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities Pubmed: 31739209
Bioengineering (Basel) Material-Dependent Formation and Degradation of Bone Matrix—Comparison of Two Cryogels Pubmed: 32517006
BioMed Research International Comparable Effects of Strontium Ranelate and Alendronate Treatment on Fracture Reduction in a Mouse Model of Osteogenesis Imperfecta 33506016
PHARMACEUTICAL BIOLOGY Effects of icariin on the fracture healing in young and old rats and its mechanism 34511043
Evid Based Complement Alternat Med Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway 34512780
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