ELISA Kit for 8-Hydroxydeoxyguanosine (8-OHdG)
8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG
- Product No.CEA660Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range74.07-6,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 26.81pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of 8-Hydroxydeoxyguanosine (8-OHdG).
No significant cross-reactivity or interference between 8-Hydroxydeoxyguanosine (8-OHdG) and analogues was observed.
Matrices listed below were spiked with certain level of 8-Hydroxydeoxyguanosine (8-OHdG) and the recovery rates were calculated by comparing the measured value to the expected amount of 8-Hydroxydeoxyguanosine (8-OHdG) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 8-Hydroxydeoxyguanosine (8-OHdG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 8-Hydroxydeoxyguanosine (8-OHdG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-Hydroxydeoxyguanosine (8-OHdG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Yonsei Med J.||Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients. Pubmed: 24142649|
|J Histochem Cytochem||Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats PubMed: 26385569|
|Diabetes/Metabolism Research and Reviews||Reduced peripheral blood mtDNA content is associated with impaired glucose‐stimulated islet β cell function in a Chinese population with different degrees of … doi:10.1002|
|Nutrition Journal||Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study Pubmed:27071648|
|Diabetology & Metabolic Syndrome||Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection pmc:PMC4855822|
|UVaDOC||Dieta hipercalórica e hipoxia intermitente crónica como modelos de obesidad y apnea obstructiva del sueño. Significado de la leptina en el control respiratorio Tesis1149-161108.pdf|
|Journal of Diabetes Mellitus||The Activity of Serum 8-Iso-Prostaglandin F2α as Oxidative Stress Marker in Patients with Diabetes Mellitus Type 2 and Associated Dyslipidemic Hyperglycemia profile:Wesam_Nasef3|
|Indian Journal of Hematology and Blood Transfusion||Oleuropein Ameliorates Cisplatin-induced Hematological Damages Via Restraining Oxidative Stress and DNA Injury article:10.1007|
|Journal of Diabetes Research||Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-TemporalStudy: Oxidative Stress and Diabetic Salivary Glands. pubmed:27478848|
|Front Physiol.||Oxidative Modification in the Salivary Glands of High Fat-Diet Induced Insulin Resistant Rats. pubmed:28184199|
|Archives of Oral Biology||Chronic high-protein diet induces oxidative stress and alters the salivary gland function in rats pubmed:28926744|
|BioMed Research International||Oxidative modification of biomolecules in the non-stimulated and stimulated saliva of patients with morbid obesity treated with bariatric surgery 10.1155:2017|
|Oncotarget||Chemopreventive effects of atractylenolide II on mammary tumorigenesis via activating Nrf2-ARE pathway pubmed:29100404|
|International Journal of Molecular Sciences||Antioxidant Defence, Oxidative Stress and Oxidative Damage in Saliva, Plasma and Erythrocytes of Dementia Patients. Can Salivary AGE be a Marker of Dementia? pubmed:29053628|
|Antioxidants and Redox Signaling||Chemokine Receptor CXCR4 Plays a Crucial Role in Mediating Oxidative StressInduced Podocyte Injury 10.1089:ars.2016.6758|
|Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats ISSN:1940-5901/IJCEM0048822|
|Biological Trace Element Research||Protective Effect of Origanum Oil on Alterations of Some Trace Elements and Antioxidant Levels Induced by Mercuric Chloride in Male Rats pubmed:28616770|
|HAYATI Journal of Biosciences||Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult SpragueeDawley Rats 10.1016/j.hjb.2017.08.007|
|Scientific Reports||Risk of glomerular filtration rate decline in patients with hypertrophic cardiomyopathy and obstructive sleep apnoea pubmed:29234143|
|Journal of Pain Research||Sex differences in complex regional pain syndrome type I (CRPS-I) in mice pubmed:28831269|
|HAYATI Journal of Biosciences||Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult Sprague–Dawley Rats 10.1016/j.hjb.2017.08.007|
|Oxidative Medicine and Cellular Longevity||Effect of N-Acetylcysteine on Antioxidant Defense, Oxidative Modification, and Salivary Gland Function in a Rat Model of Insulin Resistance Pubmed:29636851|
|Oxidative medicine and cellular longevity||Exposure to Ti4Al4V Titanium Alloy Leads to Redox Abnormalities, Oxidative Stress, and Oxidative Damage in Patients Treated for Mandible Fractures Pubmed:30013720|
|Journal of Cardiovascular Development and Disease||Oxidative DNA Damage and Carotid Intima Media Thickness as Predictors of Cardiovascular Disease in Prediabetic Subjects Pubmed:29518984|
|Reproductive Biology||Anti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspring Pubmed:30005909|
|Oxidative medicine and cellular longevity||Redox Balance, Antioxidant Defense, and Oxidative Damage in the Hypothalamus and Cerebral Cortex of Rats with High Fat Diet-Induced Insulin Resistance|
|Journal of Pharmacological Reports||Journal of Pharmacological Reports Pubmed: 27489887|
|Diabetes & Metabolic Syndrome: Clinical Research & Reviews||Cross-sectional correlates of oxidative stress and inflammation with glucose intolerance in prediabetes|
|BioMed Research International||Oral Administration of Lactobacillus delbrueckii during the Suckling Phase Improves Antioxidant Activities and Immune Responses after the Weaning Event in a Piglet …|
|Oxidative Medicine and Cellular Longevity||Luteolin Attenuates Cardiac Ischemia/Reperfusion Injury in Diabetic Rats by Modulating Nrf2 Antioxidative Function|
|Oxidative Medicine and Cellular Longevity||Dendrobium Officinale Polysaccharides Protect against MNNG-Induced PLGC in Rats via Activating the NRF2 and Antioxidant Enzymes HO-1 and NQO-1|
|International Journal of Pharmaceutical Research & Allied Sciences||Prophylactic Impact of Beta vulgaris L in Ameliorating Cyclosporine A-Induced Hepatotoxicity in Rats.|
|Journal of Diabetes and Metabolism||Assessment of 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as a Diagnostic Marker for Diabetic Nephropathy in Type 1 Egyptian Diabetic Patients|
|Journal of Food Biochemistry||Dietary supplementation of barley and/or dates attenuate hypercholesterolemic‐induced endometrial dysfunction in Wistar albino rats via alleviation of apoptotic … Pubmed: 31373029|
|environmental science and pollution research||Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains Pubmed: 31468353|
|Oxidative Medicine and Cellular Longevity||Leukocyte Telomere Length Independently Predicts 3-Year Diabetes Risk in a Longitudinal Study of Chinese Population Pubmed: 32215181|
|Journal of Clinical Medicine||Dysfunction of Salivary Glands, Disturbances in Salivary Antioxidants and Increased Oxidative Damage in Saliva of Overweight and Obese Adolescents Pubmed: 32079369|
|Frontiers in Pharmacology||Hydrogen Sulfide Attenuates Particulate Matter-Induced Emphysema and Airway Inflammation Through Nrf2-Dependent Manner Pubmed: 32116706|
|Odontology||Salivary 8-hydroxyguanosine levels in smokers and non-smokers with chronic periodontitis Pubmed: 32065311|
|FREE RADICAL RESEARCH||Negative association between antioxidant vitamin intake and non-alcoholic fatty liver disease in Chinese non-diabetic adults: mediation models involving superoxide … Pubmed: 32985285|
|JOURNAL OF FOOD SCIENCE||Lycopene protects neuroblastoma cells against oxidative damage via depression of ER stress Pubmed: 32885410|
|Medicine||Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study Pubmed: 32756181|
|Lipids Health Dis||Triglyceride is independently correlated with insulin resistance and islet beta cell function: a study in population with different glucose and lipid metabolism … Pubmed: 32487177|
|Theranostics||Rescuing Dicer expression in inflamed colon tissues alleviates colitis and prevents colitis-associated tumorigenesis Pubmed: 32483416|
|Journal of Clinical Medicine||Systemic Redox Imbalance in Patients with Chronic Granulomatous Disease Pubmed: 32397350|
|Oxidative Medicine and Cellular Longevity||NAC Supplementation of Hyperglycemic Rats Prevents the Development of Insulin Resistance and Improves Antioxidant Status but Only Alleviates General … Pubmed: 33274008|
|OXIDATIVE MEDICINE AND CELLULAR LONGEVITY||Potential Protective Effect of Dietary Intake of Non-α-Tocopherols on Cellular Aging Markers Mediated by Tumor Necrosis Factor-α in Prediabetes: A Cross … Pubmed: 32509152|
|oxidative medine cellular longevity||Relationship between Decreased Serum Superoxide Dismutase Activity and Metabolic Syndrome: Synergistic Mediating Role of Insulin Resistance and β … Pubmed: 32617139|
|MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS||Induction of genotoxicity and mutagenic potential of heavy metals in Thai occupational workers Pubmed: 32928371|
|Journal of Functional Foods||Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets|
|Journal of Diabetes Research||Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China|
|The potential protective role of gamma-irradiated vaccine versus Punica granatum treatment against murine trichinellosis|
|Mutat Res Gen Tox En|
|Electroacupuncture ameliorates motor dysfunction via inhibiting p66Shc-mediated oxidative stress and endoplasmic reticulum stress in rats with spinal cord …|
|Journal of Skin and Stem Cell||8-OHdG and hOGG1 as Oxidative DNA Damage Markers in Acne Vulgaris Patients Under Isotretinoin Treatment|
|Redox Biol||Biomarkers of nucleic acid oxidation¨Ca summary state-of-the-art 33579665|
|Theranostics||Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage 33897894|
|Exp Ther Med||Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats 34093766|
|chemosphere||Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective 33662726|
|Food Funct.||Sestrin2 is involved in Nrf2-regulated antioxidative signaling pathway in luteolin-induced prevention of the diabetic rat heart from ischemia/reperfusion injury 33900303|
|Aquaculture Reports||Growth performance, antioxidant capacity, tissue fatty acid composition and lipid metabolism of juvenile green mud crab Scylla paramamosain in response to?¡|
|Ann Behav Med||Role of Yoga and Its Plausible Mechanism in the Mitigation of DNA Damage in Type-2 Diabetes: A Randomized Clinical Trial 34180500|
|Cancer Manag Res||Association of Tumour Microenvironment with Protein Glycooxidation, DNA Damage, and Nitrosative Stress in Colorectal Cancer 34408493|
|The Journal of Clinical Endocrinology & Metabolism||High Hemoglobin glycation index is associated with telomere attrition independent of HbA1c, mediating by TNFα 34562085|
|Ecotoxicol Environ Saf||Chromium induced neurotoxicity by altering metabolism in zebrafish larvae 34781135|
|Toxins||Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy 34437430|
|J Int Med Res||Body mass index is associated with Helicobacter pylori infection and increased oxidative DNA damage in an obese population Pubmed:35209724|
|Ann Transplant||Biochemical and Molecular Studies of Different Parameters as Markers for Nephropathy in Type 1 Diabetic Patients Pubmed:34751188|
|Oxidative Medicine and Cellular Longevity||Blockage of Fibronectin 1 Ameliorates Myocardial Ischemia/Reperfusion Injury in Association with Activation of AMP-LKB1-AMPK Signaling Pathway Pubmed:35602095|
|Catalog No.||Related products for research use of Pan-species (General) Organism species||Applications (RESEARCH USE ONLY!)|
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