ELISA Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI)



This assay has high sensitivity and excellent specificity for detection of Cross Linked C-Telopeptide Of Type I Collagen (CTXI).
No significant cross-reactivity or interference between Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-95 88
EDTA plasma(n=5) 92-105 95
heparin plasma(n=5) 78-96 89


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type I Collagen (CTXI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type I Collagen (CTXI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 94-102% 96-104% 81-103%
EDTA plasma(n=5) 90-99% 87-96% 86-96% 78-93%
heparin plasma(n=5) 92-101% 85-97% 87-96% 94-102%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
journal of bone and mineral research Atp6v0d2 Is an Essential Component of the Osteoclast-Specific Proton Pump That Mediates Extracellular Acidification in Bone Resorption PubMed: PMC2672205
Bone Borage and fish oils lifelong supplementation decreases inflammation and improves bone health in a murine model of senile osteoporosis PubMed: 21664309
Trials Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial. Trials: 1745-6215
Journal of Biological Chemistry TRAF family member-associated NF-κB activator (TANK) is a negative regulator of osteoclastogenesis and bone formation PubMed: 22773835
The Journal of Clinical Endocrinology & Metabolism A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular Risk PubMed: PMC3462931
Endocrinology Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat Gain Endocrine: Source
Am J Physiol Endocrinol Metab. DPP IV inhibitor treatment attenuates bone loss and improves mechanical bone strength in male diabetic rats Pubmed:25053403
The American Journal of Cardiology Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry disease Pubmed:25073565
Rheumatology (Oxford). Bone resorption correlates with the frequency of CD5+ B cells in the blood of patients with rheumatoid arthritis Pubmed:25193807
XPO1/CRM1-Selective Inhibitors of Nuclear Export (SINE) reduce tumor spreading and improve overall survival in preclinical models of prostate cancer (PCa) Pubmed:25284315
Rheumatology International The presence of FCGR2B promoter or transmembrane region variant alleles leads to reduced serum IL-6 levels in rheumatoid arthritis Pubmed:25630523
The Journal of Biological Chemistry 5-azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival Pubmed:25691576
Scandinavian Journal of Clinical and Laboratory Investigation Osteocartilaginous metabolic markers change over a 3-week stage race in pro-cyclists PubMed: 26174975
AIDS Effects of renal tubular dysfunction on bone in tenofovir-exposed HIV-positive patients PubMed: 26372384
the journal of rheumatology Biomarkers in Remission According to Different Criteria in Patients with Rheumatoid Arthritis PubMed: 26472417
Int J Mol Med Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway PubMed: 26677102
the journal of biological chemistry Mutation in Osteoactivin Promotes RANKL-Mediated Osteoclast Differentiation and Survival, but Inhibits Osteoclast Function PubMed: 25837253
frontiers in endocrinology Oxytocin reverses osteoporosis in a sex-dependent manner PubMed: 26042090
PLoS One.  The RIP1–RIP3 Complex Mediates Osteocyte Necroptosis after Ovariectomy in Rats pmc:PMC4795547
J Cell Physiol Lanthanum Chloride Attenuates Osteoclast Formation and Function Via the DownreCavia (Guinea pig )lation of Rankl-Induced Nf-κb and Nfatc1 Activities Pubmed:26060084
FASEB Journal N-Homocysteinylation impairs collagen cross-linking in cystathionine β-synthase-deficient mice: a novel mechanism of connective tissue abnormalities. pubmed:27530978
Metabolism. High FGF21 levels are associated with altered bone homeostasis in HIV-1-infected patients. pubmed:28521869
journal of science and medicine in sport The effect of calcium and vitamin D supplementation on bone health of male Jockeys. pubmed:27568072
Chemico-Biological Interactions Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway pubmed:27387537
Epilepsy Research The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats pubmed:27838501
Bone Reduced femoral bone mass in both diet-induced and genetic hyperlipidemia mice pubmed:27669658
Journal of Vascular Surgery A potential role for glycated cross-links in abdominal aortic aneurysm disease. pubmed:27624703
Institute of Pharmacology Effects of long-term administration of pantoprazole on bone mineral density in young male rats. pubmed:27552060
Biomedicine & Pharmacotherapy Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats S0753332216322752
European Journal of Pharmacology Cannabidiol administration reduces sublesional cancellous bone loss in rats with severe spinal cord injury pubmed:28479140
APPLIED SPECTROSCOPY Side-Effects of Convulsive Seizures and Anti-Seizure Therapy on Bone in a Rat Model of Epilepsy pubmed:28905646
Clinical Nutrition Obesity status influences the relationship among serum osteocalcin, iron stores and insulin sensitivity pubmed:29050649
Oncotarget A novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice. pubmed:28060768
中国骨质疏松杂志 骨形成指标PINP 与骨吸收指标β-CTX 在2 型糖尿病合并骨质疏松中作用的研究. 10.3969/j.issn.1006-7108.2017.03.009
BioMed Research International Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research pubmed:29119115
Cell Death And Differentiation TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42 Pubmed:29358671
Journal of Periodontology  The role of androgens on periodontal repair in female rats Pubmed:29683499
Life Sciences Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway Pubmed:29883721
European Journal of Pharmacology The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism Pubmed:29428468
Journal of Clinical Densitometry Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX study Pubmed:30076009
Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457
Experimental and Therapeutic Medicine Liraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesis Pubmed:29805533
Experimental and Therapeutic Medicine Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice 10.3892:etm.2018.6353
Tumor Biology Dual PI3 K/mTOR inhibition reduces prostate cancer bone engraftment altering tumor-induced bone remodeling Pubmed:29687745
Sensors Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers Pubmed: 30181433
Tropical Journal of Pharmaceutical Research Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway
Bone & Joint Research Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise
Toxicol Ind Health Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed … Pubmed: 30983556
Journal of Geriatric Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors Pubmed: 30952517
Scientific Reports Significance of the Tks4 scaffold protein in bone tissue homeostasis
E The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats
Biology Open Osteocyte TSC1 promotes sclerostin secretion to restrain osteogenesis in mice Pubmed: 31088250
Journal of Periodontology Role of testosterone and androgen receptor in periodontal disease progression in female rats Pubmed: 31389012
Journal of clinical periodontology Biomarkers of oral inflammation in perinatally HIV‐infected and perinatally HIV exposed, uninfected youth Pubmed: 31385616
Bulletin of Experimental Biology and Medicine Laboratory Monitoring of Bone Tissue Remodeling after Augmentation of Impression Intraarticular Fracture with Different Types of Bone Graft Pubmed: 31630302
Molecular Therapy-Nucleic Acids AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities Pubmed: 31739209
International Journal of Environmental Research and Public Health Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats Pubmed: 31739465
International journal of molecular sciences Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients Pubmed: 31717649
Brazilian Journal of Medical and Biological Research Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals Pubmed: 31826180
Journal of Bone and Mineral Research BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodeling Pubmed: 31914211
Theranostics EDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for Osteoporosis Pubmed: 32194868
Cells Article Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous … Pubmed: 31877673
Cell RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis Pubmed: 32640190
Advanced Science A Loop‐Based and AGO‐Incorporated Virtual Screening Model Targeting AGO‐Mediated miRNA–mRNA Interactions for Drug Discovery to Rescue Bone Phenotype … Pubmed: 32670749
International Medical Journal Malaysia The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats.
Cells. Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous …
Journal of Orthopaedic Translation Qu Feng Zhi Tong capsule increases mechanical properties of cortical bone in ovariectomized rats
Research square 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice
IIUM Medical Journal Malaysia The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats
Pamukkale Tıp Dergisi Postmenapozal Tip-2 diyabetes mellitus' lu hastalarda osteoporoza bağlı kırık riskinin ve kemik Turnoveri'nin değerlendirilmesi
Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
CEA665Rb ELISA Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI) Enzyme-linked immunosorbent assay for Antigen Detection.