ELISA Kit for Beta-Endorphin (bEP)
- Product No.CEA806Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.83pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP).
No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Beta-Endorphin (bEP) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-Endorphin (bEP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-Endorphin (bEP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Experimental Dermatology||Endogenous μ-opioid peptides modulate immune response towards malignant melanoma Publish: RD10279|
|European Journal of Physiology||Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave SpringerLink: tv015l3126833315|
|International Journal of Pharmaceutical Science Invention ISSN(Online)||Effect of Ramadan fasting on endorphin and endocannabinoid level in serum, PBMC and macrophage Ijpsi: Source|
|Neurological Sciences||Evaluation of immune parameters in chronic migraine with medication overuse Pubmed:24867859|
|Brain Behav.||Cerebrospinal fluid and plasma b-endorphin levels in children with cerebral malaria. pubmed:28413714|
|Journal of Cosmet Dermatol.||Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties DOI: 10.1111/jocd.12349|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
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|CEA806Ra||ELISA Kit for Beta-Endorphin (bEP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
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