ELISA Kit for Gamma-Aminobutyric Acid (gABA)

4-Aminobutyric Acid

Specificity

This assay has high sensitivity and excellent specificity for detection of Gamma-Aminobutyric Acid (gABA).
No significant cross-reactivity or interference between Gamma-Aminobutyric Acid (gABA) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Gamma-Aminobutyric Acid (gABA) and the recovery rates were calculated by comparing the measured value to the expected amount of Gamma-Aminobutyric Acid (gABA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-99 95
EDTA plasma(n=5) 79-101 96
heparin plasma(n=5) 99-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gamma-Aminobutyric Acid (gABA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-104% 78-95% 79-98% 94-103%
EDTA plasma(n=5) 87-95% 90-98% 80-91% 80-95%
heparin plasma(n=5) 83-102% 91-98% 95-102% 95-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Toxicology and Industrial Health Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum PubMed: 21444355
Neurosci Lett Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear. PubMed: 18577424
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism PubMed: PMC3623607
Biomarker insights Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children PubMed: PMC3694825
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) PubMed: PMC3762604
Scientific Reports Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension pubmed:27917928
Journal of Neuroscience Sex differences in the glutamate signaling pathway in juvenile rats Pubmed:28861894
Scientific Reports A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme Pubmed: 30341396
Neurochemical Research Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission Pubmed: 30666488
Neuroscience Letters Differentiation of human glioblastoma U87 cells into cholinergic neuron Pubmed: 30928478
Neurochemical Journal Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism
Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats.
Experimental and Therapeutic Medicine Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsy Pubmed: 33101467
Exp Anim Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation 33828024
Neurogenetics Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases 34101060
Sci Rep Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission 34475508
J Food Biochem Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies Pubmed:35102569
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