ELISA Kit for Gamma-Aminobutyric Acid (gABA)

4-Aminobutyric Acid


This assay has high sensitivity and excellent specificity for detection of Gamma-Aminobutyric Acid (gABA).
No significant cross-reactivity or interference between Gamma-Aminobutyric Acid (gABA) and analogues was observed.


Matrices listed below were spiked with certain level of Gamma-Aminobutyric Acid (gABA) and the recovery rates were calculated by comparing the measured value to the expected amount of Gamma-Aminobutyric Acid (gABA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-99 95
EDTA plasma(n=5) 79-101 96
heparin plasma(n=5) 99-105 102


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gamma-Aminobutyric Acid (gABA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-104% 78-95% 79-98% 94-103%
EDTA plasma(n=5) 87-95% 90-98% 80-91% 80-95%
heparin plasma(n=5) 83-102% 91-98% 95-102% 95-102%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Toxicology and Industrial Health Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum PubMed: 21444355
Neurosci Lett Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear. PubMed: 18577424
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism PubMed: PMC3623607
Biomarker insights Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children PubMed: PMC3694825
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) PubMed: PMC3762604
Scientific Reports Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension pubmed:27917928
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
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