ELISA Kit for Noradrenaline (NE)
NA; NAd; Norepinephrine; Norepi
- Product No.CEA907Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range61.7-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 24.4pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! 796 US$ 796
For more details, please contact local distributors! US$ 3580
For more details, please contact local distributors! US$ 6763
For more details, please contact local distributors! US$ 55692
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Noradrenaline (NE).
No significant cross-reactivity or interference between Noradrenaline (NE) and analogues was observed.
Matrices listed below were spiked with certain level of Noradrenaline (NE) and the recovery rates were calculated by comparing the measured value to the expected amount of Noradrenaline (NE) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Noradrenaline (NE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Noradrenaline (NE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Noradrenaline (NE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Renal Failure||Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients Pubmed: 23530612|
|Acta Physiologica||Apelin-13 and APJ in paraventricular nucleus contribute to hypertension via sympathetic activation and vasopressin release in spontaneously hypertensive rats Pubmed:24995933|
|Physiological Research||Renal Sympathetic Denervation Improves Cardiac Dysfunction PubMed: 25804096|
|J Cardiovasc Pharmacol Ther||Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of 伪1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta. PubMed: 26025460|
|Neuropsychiatr Dis Treat||Catha edulis chewing effects on treatment of paranoid schizophrenic patients PubMed: 25926735|
|Journal of Caediovascular Pharmacology and Therapeutics||Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of α1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta content:21|
|Neuropharmacology||Relaxin in paraventricular nucleus contributes to sympathetic overdrive and hypertension via PI3K-Akt pathway Pubmed:26746861|
|Microchemical Journal||Determination of 17β-estradiol and noradrenaline in dog serum using surface-enhanced Raman spectroscopy and random Forest science:S0026265X16300388|
|Scientific Reports||Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesity pubmed:27694818|
|PLoS One.||Artepillin C, a Typical Brazilian Propolis-Derived Component, Induces Brown-Like Adipocyte Formation in C3H10T1/2 Cells, Primary Inguinal White Adipose Tissue-Derived Adipocytes, and Mice. pubmed:27598888|
|Cellular Physiology and Biochemistry||Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy pubmed:27889753|
|European Journal of Trauma and Emergency||Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. pubmed:28070608|
|Acta Physiologica||c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression pubmed:27439062|
|Nutrition||Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats S0899900716302350|
|Auris Nasus Larynx.||Stress hormonal changes in the brain and plasma after acute noise exposure in mice. pubmed:27496010|
|Oncotarget||Effects of renal denervation on monocrotaline induced pulmonary remodeling pubmed:28187460|
|JOURNAL OF APPLIED PHYSIOLOGY||Renal sympathetic denervation attenuates hypertension and vascular remodeling in renovascular hypertensive rats pubmed:27742806|
|Nutrition||Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats pubmed:28241996|
|Acta Physiologica||Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload pubmed:27575955|
|Proceedings of the National Academy of Sciences of the United States of America (PNAS)||cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis Pubmed:29784793|
|Toxicology Reports||Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor … Pubmed:29854627|
|International Journal of Nursing Practice||Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial Pubmed:29512230|
|Acta Astronautica||A novel sympathetic neuronal GABA ergic signaling system regulates NE release to prevent ventricular arrhythmias after acute myocardial infarction|
|Journal of Taibah University for Science||Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles|
|Journal of the American College of Cardiology||Noninvasive Stereotactic Radiotherapy for Renal Denervation in a Swine Model Pubmed: 31558254|
|American Journal of Physiology-Heart and Circulatory Physiology||Exacerbated pressor and sympatho-excitatory effects of central Elabela in spontaneously hypertensive rats Pubmed: 31834836|
|European Journal of Pharmacology||Resiniferatoxin reduces ventricular arrhythmias in heart failure via selectively blunting cardiac sympathetic afferent projection into spinal cord in rats Pubmed: 31811858|
|A porcine model for studying the cardiovascular consequences of high‐thoracic spinal cord injury Pubmed: 31876952|
|The American Journal of Pathology||Sympathetic Nerves Positively Regulate Eosinophil-Driven Allergic Conjunctivitis via α1-Adrenergic Receptor Signaling Pubmed: 32194050|
|pharmaceutical biology||Role of melatonin in intestinal mucosal injury induced by restraint stress in mice Pubmed: 32298156|
|Nature||Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Pubmed: 33177714|
|European Journal of Medical Research||Laparoscopic-based perivascular renal sympathetic nerve denervation: a feasibility study in a porcine model Pubmed: 32552871|
|Scientific Reports||Baroreceptor denervation reduces inflammatory status but worsens cardiovascular collapse during systemic inflammation Pubmed: 32332859|
|bioRxiv||Baroreceptor denervation reduces inflammatory status and worsens cardiovascular collapse during systemic inflammation|
|Blockade of adrenergic β‐receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair …|
|Cogent Medicine||Fractionation and bioassay-guided isolation of antihypertensive components of senecio serratuloides|
|PHARMACOLOGICAL RESEARCH||Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2 33352230|
|ACTA PHARMACOLOGICA SINICA||The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice 33303990|
|Mol Metab||Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D 33549845|
|International Journal of?Nanomedicine||Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡ 33762821|
|Int J Hypertens||The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models 33884205|
|Kidney Blood Press Res||Renal interstitial fibrosis is reduced in high-fat diet-induced obese pigs following renal denervation from the intima and adventitia of the renal artery 34852339|
|Hypertension||Expression of LHCGR (Luteinizing Hormone/Chorionic Gonadotrophin Receptor) in Pheochromocytomas Unveils an Endocrine Mechanism Connecting Pregnancy … Pubmed:35189708|
|Exp Eye Res||Autonomic nervous system receptor-mediated regulation of mast cell degranulation modulates the inflammation after corneal epithelial abrasion Pubmed:35421396|
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