• Featured-Product
ELISA Kit for Ovalbumin (OVA)


This assay has high sensitivity and excellent specificity for detection of Ovalbumin (OVA).
No significant cross-reactivity or interference between Ovalbumin (OVA) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Ovalbumin (OVA) and the recovery rates were calculated by comparing the measured value to the expected amount of Ovalbumin (OVA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 83-97 89
heparin plasma(n=5) 83-103 92


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ovalbumin (OVA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ovalbumin (OVA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ovalbumin (OVA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-98% 79-101% 89-101% 81-88%
EDTA plasma(n=5) 85-94% 84-92% 99-105% 92-105%
heparin plasma(n=5) 81-105% 89-102% 94-101% 84-95%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Vaccine. Non-invasive delivery of nanoparticles to hair follicles: A perspective for transcutaneous immunization Pubmed: 23290836
Cell Biology International Alphavbeta6 is required in maintaining the intestinal epithelial barrier function Pubmed:24677750
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
NPB459Ge01 Native Ovalbumin (OVA) Positive Control; Immunogen; SDS-PAGE; WB.
PAB459Ge01 Polyclonal Antibody to Ovalbumin (OVA) WB; IHC; ICC; IP.
LAB459Ge71 Biotin-Linked Polyclonal Antibody to Ovalbumin (OVA) WB; IHC; ICC.
MAB459Ge21 Monoclonal Antibody to Ovalbumin (OVA) WB; IHC; ICC; IP.
HEB459Ge High Sensitive ELISA Kit for Ovalbumin (OVA) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB459Ge ELISA Kit for Ovalbumin (OVA) Enzyme-linked immunosorbent assay for Antigen Detection.
CCB459Ge CLIA Kit for Ovalbumin (OVA) Chemiluminescent immunoassay for Antigen Detection.
KSB459Ge11 ELISA Kit DIY Materials for Ovalbumin (OVA) Main materials for "Do It (ELISA Kit) Yourself".