ELISA Kit for Lipopolysaccharide (LPS)
LOS; Lipoglycans; Lipooligosaccharide; Lipo-Oligosaccharide; Endotoxin
- Product No.CEB526Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.15ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 900
For more details, please contact local distributors! US$ 4050
For more details, please contact local distributors! US$ 7650
For more details, please contact local distributors! US$ 63000
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Lipopolysaccharide (LPS).
No significant cross-reactivity or interference between Lipopolysaccharide (LPS) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Lipopolysaccharide (LPS) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipopolysaccharide (LPS) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipopolysaccharide (LPS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipopolysaccharide (LPS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipopolysaccharide (LPS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Particle and Fibre Toxicology||Particulate matter air pollution disrupts endothelial cell barrier via calpain-mediated tight junction protein degradation PubMed: 22931549|
|Journal of Neuroinflammation||Hemoglobin induces inflammation after preterm intraventricular hemorrhage by methemoglobin formation PubMed: PMC3750409|
|Nutrition||Antidiabetic effect of Lactobacillus casei CCFM0412 in high-fat-fed, streptozotocin-induced type 2 diabetic mice ScienceDirect: S0899900714001567|
|PLoS One.||Effects of Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 on Hepatic Steatosis in Zucker Rats Pubmed:24852284|
|PLOS ONE||Immunological Activity Difference between Native Calreticulin Monomers and Oligomers Pubmed:25171171|
|Nutrition||Antidiabetic effect of Lactobacillus casei CCFM0412 on mice with type 2 diabetes induced by a high-fat diet and streptozotocin Pubmed:25102821|
|Journal of Functional Foods||Oral administration of Lactobacillus rhamnosus CCFM0528 improves glucose tolerance and cytokine secretion in high-fat-fed, streptozotocin-induced type 2 diabetic … Sciencedirect:S1756464614002138|
|summary future microbiology||Is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients? PubMed: 25865191|
|Eur J Gastroenterol Hepatol||Effects of probiotics (cultured Lactobacillus subtilis/Streptococcus faecium) in the treatment of alcoholic hepatitis: randomized-controlled multicenter study PubMed: 26302024|
|Joural of Pharmacy & Bioallied Sciences||The effects of female sexual steroids on gastric function and barrier resistance of gastrointestinal tract following traumatic brain injury PubMed: 25709342|
|Am J Clin Nutr||Fecal menaquinone profiles of overweight adults are associated with gut microbiota composition during a gut microbiota–targeted dietary intervention PubMed26016865 :|
|Am J Physiol Gastrointest||Deficiency of intestinal mucin-2 protects mice from diet-induced fatty liver disease and obesity PubMed: 26702135|
|International Immunopharmacology||Oxymatrine attenuates CCl 4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathways Pubmed:27179304|
|Clinics and Research in Hepatology and Gastroenterology||Portal vein thrombosis in cirrhosis is not associated with intestinal barrier disruption or increased platelet aggregability Pubmed:27160816|
|Nutrition||Administration of probiotic mixture DM# 1 ameliorated 5-fluorouracil-induced intestinal mucositis and dysbiosis in rats science:S0899900716300740|
|Am J Physiol Gastrointest Liver Physiol||Inhibition of cyclooxygenase-2 alleviates liver cirrhosis via improvement of the dysfunctional Cavia (Guinea pig )t-liver axis in rats Pubmed:27056726|
|molecular nutrition & food research||Food combination based on a pre-hispanic Mexican diet decreases metabolic and cognitive abnormalities and gut microbiota dysbiosis caused by a sucrose-enriched high-fat diet in rats. pubmed:27352915|
|Nutrition||Administration of probiotic mixture DM# 1 ameliorated 5-fluorouracil–induced intestinal mucositis and dysbiosis in rats article:S0899-9007(16)30074-0|
|Biochimica et Biophysica Acta (BBA)-General Subjects||Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats pubmed:28341485|
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