ELISA Kit for Glutamic Acid (Glu)
- Product No.CES122Ge
- Organism SpeciesPan-species (General)Same name, Different species.
- Sample Typebiological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range123.5-10000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 48.2ng/mL.
- DownloadInstruction Manual
- FOB US$ 560
For negotiated price and more details, please contact local distributors! US$ 800
For negotiated price and more details, please contact local distributors! US$ 3600
For negotiated price and more details, please contact local distributors! US$ 6800
For negotiated price and more details, please contact local distributors! US$ 56000
For negotiated price and more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Glutamic Acid (Glu).
No significant cross-reactivity or interference between Glutamic Acid (Glu) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamic Acid (Glu) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamic Acid (Glu) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Glutamic Acid (Glu) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glutamic Acid (Glu) and unlabeled Glutamic Acid (Glu) (Standards or samples) with the pre-coated antibody specific to Glutamic Acid (Glu). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glutamic Acid (Glu) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glutamic Acid (Glu) in the sample.
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