High Sensitive ELISA Kit for Laminin (LN)
- Product No.HEA082Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 13.1pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 693
For more details, please contact local distributors! US$ 3119
For more details, please contact local distributors! US$ 5891
For more details, please contact local distributors! US$ 48510
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of High Sensitive Laminin (LN).
No significant cross-reactivity or interference between High Sensitive Laminin (LN) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive Laminin (LN) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Laminin (LN) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Laminin (LN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Laminin (LN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Laminin (LN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Stem Cell Research Therapy||Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658|
|PLoS One.||Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592|
|Clin Exp Metastasis.||Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progression Pubmed:24807734|
|European Journal of Obstetrics & Gynecology and Reproductive Biology||Investigation?of?diagnostic?potentials?of?nine?different?biomarkers?in?endometriosis. Pubmed:24813083|
|Archives of Plastic Surgery||Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas PubMed: 26430624|
|Canadian Journal of Physiology and Pharmacology||Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cells Doi: Abs|
|MOLECULAR AND CLINICAL ONCOLOGY||Clinical significance of serum laminin and typeâIV collagen levels in cutaneous melanoma patients Pubmed:27330797|
|Am J Transplant.||Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation. pubmed:27456745|
|Asian Pacific Journal of Cancer Prevention||Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Response pubmed:29172262|
|Diabetes||Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in Adipocytes Pubmed:29986925|
|European Food Research and Technology||Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis 10.1007:s00217-018-3119-z|
|Experimental Lung Research||High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects Pubmed: 31290711|
|Neurotherapeutics||Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair Pubmed: 31758411|
|Untersuchungen und semiquantitative Symmetrie-Analysen zur Extrazellulärmatrix von Kalbs-Stimmlippen|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|NPA082Hu01||Native Laminin (LN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA082Hu01||Polyclonal Antibody to Laminin (LN)||WB; IHC; ICC; IP.|
|LAA082Hu71||Biotin-Linked Polyclonal Antibody to Laminin (LN)||WB; IHC; ICC.|
|HEA082Hu||High Sensitive ELISA Kit for Laminin (LN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEA082Hu||ELISA Kit for Laminin (LN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA082Hu||CLIA Kit for Laminin (LN)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA082Hu01||ELISA Kit DIY Materials for Laminin (LN)||Main materials for "Do It (ELISA Kit) Yourself".|