High Sensitive ELISA Kit for Laminin (LN)



This assay has high sensitivity and excellent specificity for detection of High Sensitive Laminin (LN).
No significant cross-reactivity or interference between High Sensitive Laminin (LN) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant High Sensitive Laminin (LN) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Laminin (LN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-101 96
EDTA plasma(n=5) 84-105 88
heparin plasma(n=5) 98-105 102


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Laminin (LN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Laminin (LN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Laminin (LN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-105% 96-104% 90-104% 82-104%
EDTA plasma(n=5) 81-96% 97-105% 96-103% 93-102%
heparin plasma(n=5) 89-103% 80-95% 93-105% 85-101%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Stem Cell Research Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592
Clin Exp Metastasis. Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progression Pubmed:24807734
European Journal of Obstetrics &amp; Gynecology and Reproductive Biology Investigation?of?diagnostic?potentials?of?nine?different?biomarkers?in?endometriosis. Pubmed:24813083
Archives of Plastic Surgery Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas PubMed: 26430624
Canadian Journal of Physiology and Pharmacology Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cells Doi: Abs
MOLECULAR AND CLINICAL ONCOLOGY Clinical significance of serum laminin and type‑IV collagen levels in cutaneous melanoma patients Pubmed:27330797
Am J Transplant. Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation. pubmed:27456745
Asian Pacific Journal of Cancer Prevention Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Response pubmed:29172262
Diabetes Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in Adipocytes Pubmed:29986925
European Food Research and Technology Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis 10.1007:s00217-018-3119-z
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Neurotherapeutics Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair Pubmed: 31758411
Untersuchungen und semiquantitative Symmetrie-Analysen zur Extrazellulärmatrix von Kalbs-Stimmlippen
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