High Sensitive ELISA Kit for Malondialdehyde (MDA)

Specificity

This assay has high sensitivity and excellent specificity for detection of High Sensitive Malondialdehyde (MDA).
No significant cross-reactivity or interference between High Sensitive Malondialdehyde (MDA) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of High Sensitive Malondialdehyde (MDA) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Malondialdehyde (MDA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-92 88
EDTA plasma(n=5) 91-99 95
heparin plasma(n=5) 81-95 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Malondialdehyde (MDA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Malondialdehyde (MDA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Malondialdehyde (MDA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-95% 95-105% 80-95% 96-103%
EDTA plasma(n=5) 79-92% 92-99% 84-95% 90-104%
heparin plasma(n=5) 78-92% 97-104% 78-92% 94-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Cell stress chaperones Lipopolysaccharide pretreatment protects against ischemia/reperfusion injury via increase of HSP70 and inhibition of NF-κB PubMed: 21562784
Liver transplantation Effect of intermittent hepatic inflow occlusion with the Pringle maneuver during donor hepatectomy in adult living donor liver transplantation with right hemiliver grafts: a prospective, randomized controlled study PubMed: 21837746
Journal of Molecular Neuroscience Formaldehyde Impairs Learning and Memory Involving the Disturbance of Hydrogen Sulfide Generation in the Hippocampus of Rats PubMed: 23108488
Hepatogastroenterology The amelioration of AH by abstinence and the attenuation of oxidative stress PubMed: 21940381
Clinical and Experimental Hypertension High salt intake accelerated cardiac remodeling in spontaneously hypertensive rats: time window of left ventricular functional transition and its relation to salt-loading doses PubMed: 21529316
AJBM Effect of caffeic acid on doxorubicin induced cardiotoxicity in rats Ajbm: Source
Yonsei Med J. Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients. Pubmed: 24142649
Cardiovasc Pathol.? Chronic thromboembolic pulmonary hypertension is not associated with iron overload Pubmed:25449047
Bosn J Basic Med Sci. Oxidative stress status in elite athletes engaged in different sport disciplines Pubmed:Pmc4333958
Neurochemical Research Role of Aldehyde Dehydrogenase 2 in 1-Methy-4-Phenylpyridinium Ion-Induced Aldehyde Stress and Cytotoxicity in PC12 Cells Pubmed:25005621
Stroke.? Ischemic postconditioning relieves cerebral ischemia and reperfusion injury through activating T-LAK cell-originated protein kinase/protein kinase B pathway in rats. Pubmed:25013016
Mol Med Rep Metformin alleviates high glucose PubMed: 25760137
Mediators Inflamm. Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic Syndrome PubMed: 26538831
PLOS ONE BDNF-TrkB Pathway Mediates Neuroprotection of Hydrogen Sulfide against Formaldehyde-Induced Toxicity to PC12 Cells PubMed: 25749582
Mediators of Inflammation Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic Syndrome PubMed: 26538831
Sci Rep Mitochondrial respiratory dysfunctions of blood mononuclear cells link with cardiac disturbance in patients with early-stage heart failure PubMed: 26018291
Nutrition Obstructive sleep apnea is independently associated with inflammation and insulin resistance, but not with blood pressure, PubMed: 26429654
Med Sci Monit Puerarin Attenuates Anoxia/Reoxygenation Injury Through Enhancing Bcl-2 Associated Athanogene 3 Expression, a Modulator of Apoptosis and Autophagy Pubmed:27011313
BMC Complement Altern Med Mechanism (s) of action underlying the gastroprotective effect of ethyl acetate fraction obtained from the crude methanolic leaves extract of Muntingia calabura Pubmed:26912079
Toxicology Mechanisms and Methods Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanism pubmed:27599793
International Journal of Neuropsychopharmacology Hydrogen Sulfide Ameliorates Homocysteine-Induced Cognitive Dysfunction by Inhibition of Reactive Aldehydes Involving Upregulation of ALDH2 pubmed:27988490
Revista de Chimie -Bucharest Comparative Analysis of Some Antioxidant Markers in Periodontal Disease pdf:MARTU%207%2016.pdf
打不开网页 Influence of Supplementing Diet with Microalgae (Schizochytrium limacinum) on Growth and Metabolism of Lambs during the Summer 2 180761
Cellular Physiology and Biochemistry Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy pubmed:27889753
Chinese Journal of Integrative Medicine Panax notoginseng saponins protect kidney from diabetes by up-regulating silent informationregulator 1 and activating antioxidant proteins in rats. pubmed:26712211
Experimental Cell Research Hydrogen sulfide inhibits MPP+-induced aldehyde stress and endoplasmic reticulum stress inPC12 cells: involving upregulation of BDNF. pubmed:27641114
Anim Reprod Sci. Transcriptional abundance of antioxidant enzymes in endometrium and their circulating levels in Zebu cows with and without uterine infection. pubmed:28007408
Journal of Cancer Research and Treatment Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines jcrt:5
Clin Exp Pharmacol Physiol. Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688
Turkish Journal of Veterinary and Animal Sciences Influence of supplementing diet with microalgae (Schizochytrium limacinum) on growth and metabolism in lambs during the summer 795296
Oncotarget Hydrogen sulfide attenuates chronic restrain stress-induced cognitive impairment by upreglulation of Sirt1 in hippocampus pubmed:29245987
Experimental and Therapeutic Medicine Resveratrol attenuates type 2 diabetes mellitus by mediating mitochondrial biogenesis and lipid metabolism via Sirtuin type 1 10.3892/etm.2017.5400
Pharmacological Reports Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without posttraumatic stress disorder 10.1016/j.pharep.2017.08.008
Journal of Pain Research Sex differences in complex regional pain syndrome type I (CRPS-I) in mice pubmed:28831269
Environmental toxicology and pharmacology Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stress pubmed:28654832
Frontiers in Microbiology Burn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress. pubmed:28702016
Ecotoxicology and Environmental Safety Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oil Pubmed:29660615
Biomed Research International Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway Pubmed:29770336
Toxicologic Pathology Gold Nanoparticle Toxicity in Mice and Rats: Species Differences Pubmed:29742986
Molecular Medicine Reports Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473
Molecular Medicine Reports  Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats Pubmed:29956781
Molecular Medicine Reports Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathway Pubmed:29620209
Oncology Letters Hydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of … Pubmed:29928398
Human and Ecological Risk Assessment: An International Journal Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails 10.1080:10807039.2018.1460800
Experimental and Therapeutic Medicine Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway Pubmed:30116359
Biochemistry and Biotechnology Research Lead-induced increase in erythrocyte osmotic fragility and malondialdehyde concentration in Wistar rats: Chemo-protective effects of flavonoid mixture 2018/1/17-021.pdf
BMC Pharmacology and Toxicology Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis Pubmed:29321061
Medical Journal Armed Forces India Are thalassemia patients oxidatively challenged? 10.1016:j.mjafi.2017.12.002
Arquivos Brasileiros de Cardiologia Serum Uric Acid Levels are Associated with Cardiometabolic Risk Factors in Healthy Young and Middle-Aged Adults Pubmed: 30328946
Clinical and Translational Oncology Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1 Pubmed: 30284230
BMC Complementary and Alternative Medicine  Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents Pubmed: 28095910
Journal of Pharmacological Sciences Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783
Biochemical and Biophysical Research Communications Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats Pubmed: 30454888
Journal of Integrative Medicine Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats Doi: 10.1016/j.joim.2018.12.002
Stem Cells Translational Medicine Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats Pubmed: 30569668
Evidence-Based Complementary and Alternative Medicine Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway
Examination of Oxidative Stress Markers in Women with Postmenopausal Osteoporosis
Acta clinica Croatica The Relationship Between Serum Level of Malondialdehyde and Progression of Colorectal Cancer
Journal of Pediatric Surgery The effects of hydrogen-rich saline solution on intestinal anastomosis performed after intestinal ischemia reperfusion injury
European Journal of Biomedical and Pharmaceutical Sciences European Journal of Biomedical AND Pharmaceutical sciences
Orphanet journal of rare diseases The cardiovascular phenotype of adult patients with phenylketonuria Pubmed: 31492166
Neuropsychobiology Hydrogen Sulfide Ameliorates Cognitive Dysfunction in Formaldehyde-Exposed Rats: Involvement in the Upregulation of Brain-Derived Neurotrophic Factor Pubmed: 31550727
Alcohol. Influence of deficient nutrition on trace element status and antioxidant defense system Pubmed: 1781925
Journal of Controlled Release Ginsenoside Rg3-loaded, reactive oxygen species-responsive polymeric nanoparticles for alleviating myocardial ischemia-reperfusion injury Pubmed: 31783047
Antioxidants Phenolic Compounds and the Anti-Atherogenic Effect of Bee Bread in High-Fat Diet-Induced Obese Rats Pubmed: 31905919
Frontiers in Neuroscience Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the Hippocampus Pubmed: 32218719
PLoS One NRF2 deficiency increases obesity susceptibility in a mouse menopausal model Pubmed: 32045430
Medicina Selected Oxidative Stress Markers in Colorectal Cancer Patients in Relation to Primary Tumor Location—A Preliminary Research Pubmed: 31972987
Stem Cells International Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … Pubmed: 32300366
Sci Rep NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han population Pubmed: 33177541
LIFE SCIENCES Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathway Pubmed: 32798557
Oxidative Medicine and Cellular Longevity Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics Pubmed: 32566106
Food and Agricultural Immunology Effects of microencapsulated probiotics and plant extract on antioxidant ability, immune status and caecal microflora in Escherichia coli K88-challenged broiler …
Possible Role of Statins on the Inflammatory Biomarkers in Patients With Periodontal Disease: A Cross-Sectional Study
Tualang honey improves memory and hippocampal changes in adult offspring of prenatally stressed rats
Malaysian Journal of Medicine and Health Sciences Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING The Anticarcinogenic Effect of the Apple Polyphenol Phloretin in an Experimental Rat Model of Hepatocellular Carcinoma
Toxicology Formaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effect 33259821
Turk J Pharm Sci Tualang Honey Improves Memory and Prevents Hippocampal Changes in Prenatally Stressed Rats 33389951
Biosci Rep Endostatin attenuates heart failure via inhibiting reactive oxygen species in myocardial 32686821
Stem Cell Research & Therapy Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis 33413675
J Pediatr Urol Protective effects of hydrogen rich saline solution on ventral penile Mathieu type flap with penile tourniquet application in rats 33608226
Mol Med Rep Tanshinone IIA attenuates heart failure via inhibiting oxidative stress in myocardial infarction rats 33786621
Amino Acids Alarin alleviated cardiac fibrosis via attenuating oxidative stress in heart failure rats 34089389
Exp Ther Med Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats 34093766
Experimental and Therapeutic Medicine MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma 33732346
The Influence of Probiotic Supplementation on Depressive Symptoms, Inflammation and Oxidative Stress Parameters and Faecal Microbiota in Patients with Depression Depending on Metabolic Syndrome Comorbidity ¨C PRO-DEMET Randomised Study Protocol 33804999
J Healthc Eng κ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB Pathway 34608407
Cell Death Discov Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats Pubmed:35022384
Sleep Breath Peripheral blood oxidative stress markers for obstructive sleep apnea—a meta-analysis Pubmed:34981298
J Dairy Sci Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver disease Pubmed:35086715
Peptides Fibroblast growth factor 12 attenuated cardiac remodeling via suppressing oxidative stress Pubmed:35304156
Peptides Targeting of midkine alleviates cardiac hypertrophy via attenuation of oxidative stress and autophagy Pubmed:35427698
Antioxidants Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice Pubmed:35740008
J Inflamm Res The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in Rats Pubmed:35645576
BioMed Research International Malondialdehyde and Neutrophil Gelatinase-Associated Lipocalin as Markers of Oxidative Stress in Small for Gestational Age Newborns from Hypertensive … Pubmed:35224102
Association of Oxidative Stress Markers with Vascular Stiffness Parameters in Patients with Diabetic Neuropathy
Stem Cell Research & Therapy Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA597Ge41 HSA Conjugated Malondialdehyde (MDA) Immunogen; SDS-PAGE; WB.
CPA597Ge11 BSA Conjugated Malondialdehyde (MDA) Immunogen; SDS-PAGE; WB.
CPA597Ge21 OVA Conjugated Malondialdehyde (MDA) Immunogen; SDS-PAGE; WB.
PAA597Ge01 Polyclonal Antibody to Malondialdehyde (MDA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA597Ge24 Monoclonal Antibody to Malondialdehyde (MDA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA597Ge23 Monoclonal Antibody to Malondialdehyde (MDA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA597Ge22 Monoclonal Antibody to Malondialdehyde (MDA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA597Ge21 Monoclonal Antibody to Malondialdehyde (MDA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA597Ge ELISA Kit for Malondialdehyde (MDA) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA597Ge High Sensitive ELISA Kit for Malondialdehyde (MDA) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA597Ge Mini Samples ELISA Kit for Malondialdehyde (MDA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA597Ge Multiplex Assay Kit for Malondialdehyde (MDA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA597Ge11 ELISA Kit DIY Materials for Malondialdehyde (MDA) Main materials for "Do It (ELISA Kit) Yourself".