Instant ELISA Kit for Progesterone (PG)
- Product No.IEA459Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typen/a
- Test MethodCompetitive Inhibition
- Assay Length1h, 10min
- Detection Rangen/a
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 1194
For more details, please contact local distributors! US$ 5373
For more details, please contact local distributors! US$ 10149
For more details, please contact local distributors! US$ 83580
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Instant Progesterone (PG).
No significant cross-reactivity or interference between Instant Progesterone (PG) and analogues was observed.
Matrices listed below were spiked with certain level of Instant Progesterone (PG) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Progesterone (PG) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Progesterone (PG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Progesterone (PG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Progesterone (PG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Fertility and Sterility||Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356|
|Am J Obstet Gynecol.||Prevention of preterm birth by progestational agents: what are the molecular mechanisms? Pubmed: 23433326|
|Placenta||The role of Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS) in proliferation, apoptosis and hormone secretion of trophoblast cells Pubmed: 23966103|
|Veterinary Immunology and Immunopathology||Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period Pubmed:27090626|
|Clinical and Experimental Hypertension||Assessment of ADMA, estradiol, and progesterone in severe preeclampsia Pubmed:27152507|
|Scientific Reports||The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization pubmed:27876896|
|Scientific Reports||Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373|
|33||Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389|
|Toxicological Sciences||Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384|
|Molecular Neurobiology||Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology. pubmed:27730513|
|Sci Rep||Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267|
|Interact Cardiovasc Thorac Surg||Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats pubmed:29049608|
|Toxicological Sciences||Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction pubmed:28973666|
|Life Sciences||The anorectic agent, lorcaserin, disturbs estrous cyclicity and produces endometrial hyperplasia without affecting ovarian population in female rats pubmed:28599864|
|Placenta||Progesterone attenuates hypertension and autoantibody levels to the angiotensin II type 1 receptor in response to elevated cadmium during pregnancy 10.1016:j.placenta.2017.12.004|
|Nanotheranostics||The biocompatibility studies of polymer dots on pregnant mice and fetuses pubmed:29071192|
|DARU Journal of Pharmaceutical Sciences||Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999|
|Toxicological Sciences||Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci|
|Frontiers in Molecular Neuroscience||Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355|
|Medical Science Monitor||Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian … Pubmed:29674607|
|Oncology Letters||Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes … 10.3892:ol.2018.9165|
|Biomedicine & Pharmacotherapy||Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135|
|Journal of Endocrinology||Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146|
|Stem Cell Research & Therapy||Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243|
|Journal of Cellular Physiology||Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression Pubmed: 30671954|
|Experimental and Therapeutic Medicine||Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary|
|Experimental Neurology||Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause Pubmed: 31377402|
|Molecular reproduction and development||Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries Pubmed: 31490589|
|Journal of Ovarian Research||Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase Pubmed: 31722718|
|Chemosphere||Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Pubmed: 31809938|
|Biosensors & Bioelectronics||An antibody-aptamer sandwich cathodic photoelectrochemical biosensor for the detection of progesterone Pubmed: 32339148|
|JOURNAL OF MOLECULAR NEUROSCIENCE||Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of Treatment Pubmed: 33159671|
|Chemosphere||Prenatal exposure of female mice to perﬂuorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … Pubmed: 33131727|
|ARCHIVES OF GYNECOLOGY AND OBSTETRICS||MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance Pubmed: 32757043|
|The Journal of Physiology||Guoqi Liu1, Juan Li2, 3, Peng Cui2, Susanne Lager5, Amanda Nancy Sferruzzi-Perri6, Wei Li1, Xiao-ke Wu1, Yanhua Han1, Mats Brännström7, Linus R Shao2 … Pubmed: 31206177|
|BioMed Research International||Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488|
|DOMESTIC ANIMAL ENDOCRINOLOGY||Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells Pubmed: 32739762|
|Journal of Reproductive Immunology||Implantation associated changes in expression profile of indoleamine-2, 3-dioxygenase 1, Th1-Th2 cytokines and interferon-stimulated genes on neutrophils and … Pubmed: 32846354|
|Iranian Journal of Basic Medical Sciences||Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS|
|Improving reproductive efficiency through the supplementation of mustard oil, poly-herbal mixture and butyric acid during the periparturient period in Sahiwal …|
|Ecotoxicol Environ Safe||A preliminary study on the mechanism of the neurosteroid-mediated ionotropic receptor dysfunction in neurodevelopmental toxicity induced by?¡ 33862428|
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