Instant ELISA Kit for Prostaglandin H2 (PGH2)
- Product No.IEA612Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typen/a
- Test MethodCompetitive Inhibition
- Assay Length1h, 10min
- Detection Rangen/a
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Instant Prostaglandin H2 (PGH2).
No significant cross-reactivity or interference between Instant Prostaglandin H2 (PGH2) and analogues was observed.
Matrices listed below were spiked with certain level of Instant Prostaglandin H2 (PGH2) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Prostaglandin H2 (PGH2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Prostaglandin H2 (PGH2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Prostaglandin H2 (PGH2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Prostaglandin H2 (PGH2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Proceedings of the National Academy of Sciences of USA||Tissue-specific contributions of Tmem79 to atopic dermatitis and mast cell-mediated histaminergic itch Pubmed: 30463955|
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