Multiplex Assay Kit for FMS Like Tyrosine Kinase 3 (Flt3) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD135; STK1; FLK2; Fms-Related Tyrosine Kinase 3; Stem Cell Tyrosine Kinase 1; FL Cytokine Receptor; Fetal Liver Kinase-2

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of FMS Like Tyrosine Kinase 3 (Flt3) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between FMS Like Tyrosine Kinase 3 (Flt3) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant FMS Like Tyrosine Kinase 3 (Flt3) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of FMS Like Tyrosine Kinase 3 (Flt3) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-99 94
EDTA plasma(n=5) 78-89 83
heparin plasma(n=5) 87-101 92
sodium citrate plasma(n=5) 88-96 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 (Flt3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 (Flt3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FMS Like Tyrosine Kinase 3 (Flt3) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-101% 80-98% 90-97% 89-103%
EDTA plasma(n=5) 86-98% 94-103% 83-101% 80-88%
heparin plasma(n=5) 80-98% 78-91% 93-101% 84-104%
sodium citrate plasma(n=5) 93-101% 94-103% 92-101% 90-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:Flt3) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Frontiers in physiology Alpha-1 Antitrypsin Prevents the Development of Preeclampsia Through Suppression of Oxidative Stress pubmed:27303303
PLoS One Evaluation of surfactant proteins A, B, C, and D in articular cartilage, synovial membrane and synovial fluid of healthy as well as patients with osteoarthritis and … Pubmed: 30235245
Bioscience Reports N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation. Doi: 10.1042/BSR20180858
Molecular Neurobiology Elevated levels of multifunctional surfactant proteins in cerebrospinal fluid are associated with signs of increased cerebrospinal fluid flow in cranial magnetic … Doi: 10.1007/s12035-017-0835-5
Cancers Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia Pubmed: 31718075
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA039Hu01 Recombinant FMS Like Tyrosine Kinase 3 (Flt3) Positive Control; Immunogen; SDS-PAGE; WB.
PAA039Hu01 Polyclonal Antibody to FMS Like Tyrosine Kinase 3 (Flt3) WB; IHC; ICC; IP.
MAA039Hu22 Monoclonal Antibody to FMS Like Tyrosine Kinase 3 (Flt3) WB; IHC; ICC; IP.
SEA039Hu ELISA Kit for FMS Like Tyrosine Kinase 3 (Flt3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA039Hu Multiplex Assay Kit for FMS Like Tyrosine Kinase 3 (Flt3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.