Multiplex Assay Kit for Fibrinogen (FG) ,etc. by FLIA (Flow Luminescence Immunoassay)

F1; FI; Fbg; Coagulation Factor I

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibrinogen (FG) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Fibrinogen (FG) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibrinogen (FG) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibrinogen (FG) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-95 88
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 88-97 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen (FG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen (FG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibrinogen (FG) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-95% 90-98% 80-101% 90-104%
EDTA plasma(n=5) 85-94% 78-105% 79-91% 80-94%
heparin plasma(n=5) 81-92% 80-92% 78-97% 79-92%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FG) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880
VETERINARSKI ARHIV Biomarkers of ketosis in dairy cows at postparturient period: acute phase proteins and pro-inflammatory cytokines doi: 10.24099/vet.arhiv.160126c
VETERINARSKI ARHIV Biomarkeri ketoze u mliječnih krava u postpartalnom razdoblju: proteini akutne faze i proupalni citokini doi: 10.24099/vet.arhiv.160126c
Spike protein up-regulates inflammatory axis of both thromboinflammation and leukotriene in severe COVID-19
Toxicol Appl Pharmacol CREB1 protects against the renal injury in a rat model of kidney stone disease and calcium oxalate monohydrate crystals-induced injury in NRK-52E cells 33421503
large animal review Influence of niacin application on inflammatory parameters, non-esterified fatty acids and functional status of liver in cows during early lactation
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
NPA193Mu01 Native Fibrinogen (FG) Positive Control; Immunogen; SDS-PAGE; WB.
SEA193Mu ELISA Kit for Fibrinogen (FG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA193Mu Multiplex Assay Kit for Fibrinogen (FG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.