Multiplex Assay Kit for Galectin 3 (GAL3) ,etc. by FLIA (Flow Luminescence Immunoassay)

LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Galectin 3 (GAL3) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Galectin 3 (GAL3) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Galectin 3 (GAL3) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Galectin 3 (GAL3) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-101 94
EDTA plasma(n=5) 86-94 90
heparin plasma(n=5) 81-96 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 3 (GAL3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 3 (GAL3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Galectin 3 (GAL3) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-102% 89-101% 98-105% 97-105%
EDTA plasma(n=5) 82-93% 93-101% 79-101% 95-102%
heparin plasma(n=5) 98-105% 87-96% 78-98% 83-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:GAL3) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Clinica Chimica Acta Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population Pubmed:25578395
Scientific Reports Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153
Int J Mol Sci The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599
Scientifi Reportcs Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices PubMed: 26582585
Clin Chim Acta Galectin-1 and galectin-4: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population PubMed: 25578396
JOURNAL OF THE AMERICAN SOCIETY OF HYPERTENSION Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4. pubmed:28431936
昆明医科大学学报 半乳糖凝集素3 在COPD 和肺癌中的表达及其意义. 2095610X
Archives of Medical Science Can we define migraine patients with blood high-sensitivity C-reactive protein and galectin-3 levels in the emergency department? publication:305625095
Biosensors & Bioelectronics A sensitive sandwich-type immunosensor for the detection of galectin-3 based on N-GNRs-Fe-MOFs@AuNPs nanocomposites and a novel AuPt-methylene blue nanorod. pubmed:29096363
Scientific Reports The profile of selected single nucleotide polymorphisms in patients with hypertension and heart failure with preserved and mid-range ejection fraction. pubmed:28827564
Clinica Chimica Acta Galectin-3 in patients with coronary heart disease and atrial fibrillation Pubmed:29287900
Advances in Experimental Medicine and Biology Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease Pubmed:29429028
FASEB Journal Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922
american journal of physiology-heart and circulatory physiology Macrophage galectin-3 enhances intimal translocation of vascular calcification in diabetes mellitus Pubmed: 32216615
Cardiology Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation Pubmed: 32516780
AUSTRALIAN DENTAL JOURNAL The effect of initial periodontal treatment on gingival crevicular fluid galectin©\3 levels in participants with periodontal disease 33378559
Cell Death & Disease Trajectory modeling of endothelial-to-mesenchymal transition reveals galectin-3 as a mediator in pulmonary fibrosis 33771973
Int J Clin Pract Serum Galectin©\3 Levels in Patients with Psoriasis 34137138
Phytomedicine Zerumbone, a humulane sesquiterpene from Syringa pinnatifolia, attenuates cardiac fibrosis by inhibiting of the TGF-β1/Smad signaling pathway after myocardial … Pubmed:35405613
Medicine Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … Pubmed:35687771
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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