Multiplex Assay Kit for Collagen Type I Alpha 1 (COL1a1) ,etc. by FLIA (Flow Luminescence Immunoassay)

COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 1 (COL1a1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Collagen Type I Alpha 1 (COL1a1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Collagen Type I Alpha 1 (COL1a1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Collagen Type I Alpha 1 (COL1a1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 102
EDTA plasma(n=5) 81-93 87
heparin plasma(n=5) 97-105 101
sodium citrate plasma(n=5) 98-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagen Type I Alpha 1 (COL1a1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 83-90% 81-93% 80-97%
EDTA plasma(n=5) 80-92% 89-103% 93-105% 96-104%
heparin plasma(n=5) 78-94% 88-104% 97-105% 92-101%
sodium citrate plasma(n=5) 98-105% 78-102% 95-104% 78-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:COL1a1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology Simvastatin-loaded porous implant surfaces stimulate preosteoblasts differentiation: an in vitro study ScienceDirect: S1079210410004919
Journal of Biomedical Materials Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro study Wiley: source
European Archives of Oto-Rhino-Laryngology Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats Pubmed: 24077847
J Voice. Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study Pubmed: 24495429
Arch Oral Biol. Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. Pubmed: 24370188
Laryngoscope. Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria Pubmed:25545625
UNIVERSA MEDICINA Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture Univmed:Source
International Journal of Neuropsychopharmacology The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study. pubmed:27477355
National Natural Scientific Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396
Nephron. Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factor pubmed:28768285
Cell and Tissue Research Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and … Pubmed:29637306
International Journal of Molecular Medicine LOXL2, a copper-dependent monoamine oxidase, activates lung fibroblasts through the TGF-β/Smad pathway Pubmed: 30320382
International Journal of Molecular Sciences Association between Plasma HMGB-1 and Silicosis: A Case-Control Study Pubmed: 30558126
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945
Brazilian Journal of Pharmaceutical Sciences Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm
Ceramics International Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response
Scientific reports Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cells 33911160
Int J Mol Sci Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model Pubmed:35682677
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA350Ra01 Recombinant Collagen Type I Alpha 1 (COL1a1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA350Ra01 Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) WB; IHC; ICC; IP.
MAA350Ra21 Monoclonal Antibody to Collagen Type I Alpha 1 (COL1a1) WB; IHC; ICC; IP.
SEA350Ra ELISA Kit for Collagen Type I Alpha 1 (COL1a1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA350Ra Multiplex Assay Kit for Collagen Type I Alpha 1 (COL1a1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.