Multiplex Assay Kit for Tissue Factor Pathway Inhibitor (TFPI) ,etc. by FLIA (Flow Luminescence Immunoassay)

TFI; EPI; LACI; TFPI1; Lipoprotein-Associated Coagulation Inhibitor; Extrinsic pathway inhibitor

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Tissue Factor Pathway Inhibitor (TFPI) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Tissue Factor Pathway Inhibitor (TFPI) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Tissue Factor Pathway Inhibitor (TFPI) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Tissue Factor Pathway Inhibitor (TFPI) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
EDTA plasma(n=5) 88-98 95
heparin plasma(n=5) 89-97 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor Pathway Inhibitor (TFPI) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor Pathway Inhibitor (TFPI) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tissue Factor Pathway Inhibitor (TFPI) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
EDTA plasma(n=5) 78-92% 84-91% 86-101% 90-98%
heparin plasma(n=5) 98-105% 83-91% 80-94% 93-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:TFPI) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
South African Journal of Child Health Tissue factor pathway inhibitor in paediatric patients with nephrotic syndrome Sajch: 383
the journal of immunology Cathepsin G in Experimental Tuberculosis: Relevance for Antibacterial Protection and Potential for Immunotherapy PubMed: 26320257
Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors PubMed: 25546502
Molecular Carcinogenesis Endothelial cell‐anchored tissue factor pathway inhibitor regulates tumor metastasis to the lung in mice PubMed: 25945811
Cell Biology International Extracellular vesicles from malignant effusions induce tumor cell migration: inhibitory effect ofLMWH tinzaparin. pubmed:27435911
International Journal of Infectious Diseases Kunitz-type protease inhibitor as a vaccine candidate against schistosomiasis mansoni pubmed:29128645
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA394Mu01 Recombinant Tissue Factor Pathway Inhibitor (TFPI) Positive Control; Immunogen; SDS-PAGE; WB.
PAA394Mu01 Polyclonal Antibody to Tissue Factor Pathway Inhibitor (TFPI) WB; IHC; ICC; IP.
LAA394Mu71 Biotin-Linked Polyclonal Antibody to Tissue Factor Pathway Inhibitor (TFPI) WB; IHC; ICC.
LAA394Mu81 FITC-Linked Polyclonal Antibody to Tissue Factor Pathway Inhibitor (TFPI) WB; IHC; ICC; IF.
SEA394Mu ELISA Kit for Tissue Factor Pathway Inhibitor (TFPI) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA394Mu CLIA Kit for Tissue Factor Pathway Inhibitor (TFPI) Chemiluminescent immunoassay for Antigen Detection.
LMA394Mu Multiplex Assay Kit for Tissue Factor Pathway Inhibitor (TFPI) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA394Mu01 ELISA Kit DIY Materials for Tissue Factor Pathway Inhibitor (TFPI) Main materials for "Do It (ELISA Kit) Yourself".