Magnetic Luminex Assay Kit for 17-Hydroxyprogesterone (17-OHP)
17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogesterone
- Product No.LMA454Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range19.53-20000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.51 pg/mL.
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of 17-Hydroxyprogesterone (17-OHP).
No significant cross-reactivity or interference between 17-Hydroxyprogesterone (17-OHP) and analogues was observed.
Matrices listed below were spiked with certain level of 17-Hydroxyprogesterone (17-OHP) and the recovery rates were calculated by comparing the measured value to the expected amount of 17-Hydroxyprogesterone (17-OHP) in samples.
|Matrix||Recovery range (%)||Average(%)|
|sodium citrate plasma(n=5)||78-103||88|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 17-Hydroxyprogesterone (17-OHP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 17-Hydroxyprogesterone (17-OHP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 17-Hydroxyprogesterone (17-OHP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|sodium citrate plasma(n=5)||89-97%||86-94%||98-105%||80-105%|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|96-well plate||1||Plate sealer for 96 wells||4|
|Pre-Mixed Standard||2||Standard Diluent||1×20mL|
|Pre-Mixed Magnetic beads (22#:17-OHP)||1||Analysis buffer||1×20mL|
|Pre-Mixed Detection Reagent A||1×120μL||Assay Diluent A||1×12mL|
|Detection Reagent B (PE-SA)||1×120μL||Assay Diluent B||1×12mL|
|Sheath Fluid||1×10mL||Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 90min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
|Catalog No.||Related products for research use of Pan-species (General) Organism species||Applications (RESEARCH USE ONLY!)|
|CPA454Ge11||BSA Conjugated 17-Hydroxyprogesterone (17-OHP)||Immunogen; SDS-PAGE; WB.|
|CPA454Ge21||OVA Conjugated 17-Hydroxyprogesterone (17-OHP)||Immunogen; SDS-PAGE; WB.|
|PAA454Ge01||Polyclonal Antibody to 17-Hydroxyprogesterone (17-OHP)||ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).|
|LAA454Ge71||Biotin-Linked Polyclonal Antibody to 17-Hydroxyprogesterone (17-OHP)||WB; IHC; ICC.|
|CEA454Ge||ELISA Kit for 17-Hydroxyprogesterone (17-OHP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|IEA454Ge||Instant ELISA Kit for 17-Hydroxyprogesterone (17-OHP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|LMA454Ge||Magnetic Luminex Assay Kit for 17-Hydroxyprogesterone (17-OHP)||Magnetic Luminex Assay for Antigen Detection.|