Magnetic Luminex Assay Kit for D-Dimer (D2D) ,etc.

D 2 Dimer

(Note: Up to 8-plex in one testing reaction)


This assay has high sensitivity and excellent specificity for detection of D-Dimer (D2D) ,etc..
No significant cross-reactivity or interference between D-Dimer (D2D) ,etc. and analogues was observed.


Matrices listed below were spiked with certain level of recombinant D-Dimer (D2D) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of D-Dimer (D2D) ,etc. in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-92 89
EDTA plasma(n=5) 79-89 86
heparin plasma(n=5) 92-101 96


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Dimer (D2D) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Dimer (D2D) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of D-Dimer (D2D) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 99-105% 79-99% 83-92%
EDTA plasma(n=5) 80-102% 78-91% 78-93% 89-97%
heparin plasma(n=5) 84-95% 97-105% 87-101% 79-90%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:D2D) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.



Magazine Citations
22 Determinants of the thrombogenic potential of multiwalled carbon nanotubes ScienceDirect: S0142961211004819
Journal of Thrombosis and Haemostasis Exogenous Activated Protein C Inhibits the Progression of Diabetic Nephropathy Pubmed: 22236035
PLOS ONE Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in Mice PlosOne: Source
Haemetology Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India Pubmed: 23992124
Journal of Trauma and Acute Care Surgery Assessment of coagulopathy, endothelial injury, and inflammation after traumatic brain injury and hemorrhage in a porcine model Lww:Source
Journal of Thrombosis and Haemostasis Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cells Pubmed:25399514
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261
Medical Science Monitor Impact of β-Adrenoceptor Blockade on Systemic Inflammation and Coagulation Disturbances in Rats with Acute Traumatic Coagulopathy Pubmed:25676919
Blood Intraperitoneal administration of activated protein C prevents postsurgical adhesion band formation. PubMed: 25575539
Am J Physiol Regul Integr Comp Physiol Procoagulant and Fibrinolytic Activity after Polytrauma in Rat PubMed: 26632604
hypertension Research Upregulation of canonical transient receptor potential channel in the pulmonary arterial smooth muscle of a chronic thromboembolic pulmonary hypertension rat model PubMed: 26155749
Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors PubMed: 25546502
JCI Insight. KLF2 and KLF4 control endothelial identity and vascular integrity. pubmed:28239661
The American Society of Anesthesiologists Activated protein C drives the hyperfibrinolysis of acute traumatic coagulopathy pubmed:27841821
32 Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target pubmed:28289017
The American Journal of Pathology Inhibition of Cell Apoptosis and Amelioration of Pulmonary Fibrosis by Thrombomodulin pubmed:28739343
Atherosclerosis Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin. pubmed:28923781
28 Lactadherin Promotes Microvesicle Clearance to Prevent Coagulopathy and Improves Survival of Severe TBI Mice pubmed:29162596
Journal of Thrombosis and Thrombolysis Guided longer pulses from a diagnostic ultrasound and intraclot microbubble enhanced catheter-directed thrombolysis in vivo. pubmed:28417266
Journal of Investigative Surgery Experimental Evaluation of a New Tissue Factor-Based Topical Hemostat (TT-173) for Treatment of Hepatic Bleeding Pubmed: 30380352
Marine Drugs The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents Pubmed: 31533230
Intensive Care Med Exp Complement factor D is linked to platelet activation in human and rodent sepsis 34396466
Thromb Res NLRP3 Inflammasome contributes to endotoxin-induced coagulation Pubmed:35421682
Experimental and Therapeutic Medicine Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis Pubmed:35126715
Catalog No. Related products for research use of Equus caballus; Equine (Horse) Organism species Applications (RESEARCH USE ONLY!)
CEA506Eq ELISA Kit for D-Dimer (D2D) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA506Eq Magnetic Luminex Assay Kit for D-Dimer (D2D) ,etc. Magnetic Luminex Assay for Antigen Detection.