Multiplex Assay Kit for Platelet Derived Growth Factor Subunit A (PDGFA) ,etc. by FLIA (Flow Luminescence Immunoassay)

PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit A (PDGFA) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit A (PDGFA) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Platelet Derived Growth Factor Subunit A (PDGFA) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Platelet Derived Growth Factor Subunit A (PDGFA) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-99 95
EDTA plasma(n=5) 83-95 91
heparin plasma(n=5) 79-90 87
sodium citrate plasma(n=5) 84-96 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit A (PDGFA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit A (PDGFA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Platelet Derived Growth Factor Subunit A (PDGFA) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-104% 83-93% 80-94% 88-101%
EDTA plasma(n=5) 78-97% 95-105% 80-88% 97-105%
heparin plasma(n=5) 90-102% 85-92% 96-104% 89-97%
sodium citrate plasma(n=5) 85-93% 78-103% 82-101% 83-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PDGFA) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Canadian Journal of Physiology and Pharmacology The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats pubmed:28177680
International Immunopharmacology Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway pubmed:28521243
Open Access Macedonian Journal of Medical Sciences Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells
Prostaglandins & other Lipid Mediators Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathway Pubmed: 31698141
Ann Agric Environ Med PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis Pubmed: 32208584
Tissue Eng Regen Med Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method 34339025
Biomed Research International Thermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like Form Pubmed:35586817
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA528Mu01 Recombinant Platelet Derived Growth Factor Subunit A (PDGFA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA528Mu02 Recombinant Platelet Derived Growth Factor Subunit A (PDGFA) Positive Control; Immunogen; SDS-PAGE; WB.
APA528Mu01 Active Platelet Derived Growth Factor Subunit A (PDGFA) Cell culture; Activity Assays.
PAA528Mu01 Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA) WB; IHC; ICC; IP.
PAA528Mu02 Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA) WB; IHC; ICC; IP.
SEA528Mu ELISA Kit for Platelet Derived Growth Factor Subunit A (PDGFA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA528Mu Multiplex Assay Kit for Platelet Derived Growth Factor Subunit A (PDGFA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.